Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,526,945	C→T	E13K (GAG→AAG)	ttk ←	division inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,526,945	0	C	T	100.0%	109.3 / NA	34	E13K (GAG→AAG)	ttk	division inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

TTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACTT  >  minE/2526892‑2527011                                                      |                                                                   ttGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCg                                                   >  1:682668/1‑71 (MQ=255)   ggCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTc                                                    >  1:2426928/1‑68 (MQ=255)   ggCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCg                                                   >  1:1555178/1‑69 (MQ=255)   ggCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGc                                                  <  1:2538107/70‑1 (MQ=255)       tCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTtgc                                                                  <  1:1250963/50‑1 (MQ=255)       tCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTtgc                                                                  <  1:2936010/50‑1 (MQ=255)       tCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTtgc                                                                  <  1:2873916/50‑1 (MQ=255)        ccATCGCTGGATTTCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtt                                              <  1:74932/69‑1 (MQ=255)        ccATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtttg                                            >  1:1187237/1‑71 (MQ=255)         cATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtttgt                                           >  1:1361123/1‑71 (MQ=255)          aTCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtttg                                            <  1:2999308/69‑1 (MQ=255)            cGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGa                                                                >  1:1292355/1‑47 (MQ=255)            cGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGa                                                                >  1:2178386/1‑47 (MQ=255)             gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:2491108/71‑1 (MQ=255)             gCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGttttt                                       <  1:64844/71‑1 (MQ=255)               tGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCt                                     >  1:2227003/1‑71 (MQ=255)                ggATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTc                                                          >  1:2947348/1‑49 (MQ=255)                 gATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGc                                   >  1:2383854/1‑71 (MQ=255)                      cAGCATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCttt                                                      <  1:1808183/47‑1 (MQ=255)                         cATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTAc                           <  1:2837344/71‑1 (MQ=255)                         cATCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTAc                           <  1:2864350/71‑1 (MQ=255)                          aTCAGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTAc                           <  1:1165618/70‑1 (MQ=255)                             aGCGCCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaaaa                       <  1:1445274/71‑1 (MQ=255)                                gcCAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATAc                    >  1:815214/1‑71 (MQ=255)                                 ccAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaaaa                       <  1:2032449/67‑1 (MQ=255)                                 ccAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACaaaa                       <  1:2732702/67‑1 (MQ=255)                                  cAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATg                               <  1:886037/58‑1 (MQ=255)                                  cAGAGACTGAAGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATg                               <  1:880674/58‑1 (MQ=255)                                          gAAGTATTTCCTTGCGACGGTTCCTTTTCGCAgtttgttt                                         <  1:1279033/40‑1 (MQ=255)                                           aaGTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAAt          <  1:2638815/70‑1 (MQ=255)                                             gTATTTCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAACTACGCCTGAAAATAAg       >  1:231341/1‑71 (MQ=255)                                                tttCCTTGCGACGGTTCCTTTTCGCAGTTTGttttt                                       >  1:496981/1‑36 (MQ=255)                                                tttCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGcc                                  >  1:1792807/1‑41 (MQ=255)                                                tttCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCa                                 >  1:666713/1‑42 (MQ=255)                                                tttCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCa                                 >  1:1826431/1‑42 (MQ=255)                                                  tCCTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACtt  >  1:1705304/1‑71 (MQ=255)                                                   ccTTGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAg       >  1:682667/1‑65 (MQ=255)                                                      |                                                                   TTGGCTTCCATCGCTGGATTCCAGCATCAGCGCCAGAGACTGAAGTATTTCCTCGCGACGGTTCCTTTTCGCAGTTTGTTTTTCTGCCATGTTACAAAATACCCCTGAAAATAAGCACTT  >  minE/2526892‑2527011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑