Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,546,001	C→T	M182I (ATG→ATA)	rfaF ←	ADP‑heptose:LPS heptosyltransferase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,546,001	0	C	T	100.0%	97.8 / NA	31	M182I (ATG→ATA)	rfaF	ADP‑heptose:LPS heptosyltransferase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/16);  total (15/16)

GCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCA  >  minE/2545934‑2546062                                                                    |                                                              gCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAattatc                                                            <  1:2985868/71‑1 (MQ=255) gCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAattatc                                                            >  1:248037/1‑71 (MQ=255)  cTCCGCATAGTGGTAGTGTGGCCAGCGGTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAattatc                                                            <  1:3162440/70‑1 (MQ=255)        aTAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGtt                                                     <  1:859875/71‑1 (MQ=255)              gTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGTAAAAACCAATTATCGGACGTTCtgat                                                >  1:564470/1‑70 (MQ=255)              gTAGTGTGGCCAGCGTTTAGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCtgat                                                <  1:2344630/70‑1 (MQ=255)               tAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCtgatga                                              <  1:1720315/71‑1 (MQ=255)                 gtgtGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCtgatga                                              >  1:1725261/1‑69 (MQ=255)                 gtgtGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGaa                                             <  1:1558378/70‑1 (MQ=255)                 gtgtGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGaa                                             <  1:1650429/70‑1 (MQ=255)                    tgGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGc                                          >  1:2495223/1‑70 (MQ=255)                             ttttGCCGGACCAAACTCCGCACCCGGGCAAAATCCAATTATCGGACGTTCTGATGAAAGCGAAAAtt                                   <  1:2542599/68‑1 (MQ=255)                             ttttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAAtt                                   <  1:3088767/68‑1 (MQ=255)                             ttttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAAtt                                   <  1:852505/68‑1 (MQ=255)                               ttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGa                                 >  1:502362/1‑68 (MQ=255)                                     gACCAAACTCCGCGCCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGta                        >  1:1069747/1‑71 (MQ=255)                                     gACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTa                              <  1:865658/65‑1 (MQ=255)                                     gACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTa                              <  1:381325/65‑1 (MQ=255)                                       ccAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGtata                      >  1:223574/1‑71 (MQ=255)                                       ccAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGtata                      >  1:1672656/1‑71 (MQ=255)                                       ccAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGtata                      >  1:1517732/1‑71 (MQ=255)                                          aaCTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGa                   <  1:3019525/71‑1 (MQ=255)                                           aCTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGAt                  >  1:2323436/1‑71 (MQ=255)                                             tCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGAttt                >  1:728849/1‑71 (MQ=255)                                                gCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTa                              <  1:945006/54‑1 (MQ=255)                                                gCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTa                              <  1:1174808/54‑1 (MQ=255)                                                 cACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGa                                        <  1:881449/43‑1 (MQ=255)                                                  aCCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGa                   <  1:3023343/63‑1 (MQ=255)                                                      gggCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAAtt                                   >  1:3139563/1‑43 (MQ=255)                                                      gggCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAAtt                                   >  1:189917/1‑43 (MQ=255)                                                      gggCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTc       >  1:3177737/1‑71 (MQ=255)                                                         cAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCt    >  1:2175774/1‑71 (MQ=255)                                                           aaaaCCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCa  >  1:3167635/1‑71 (MQ=255)                                                           aaaaACAATTATCGGAAGTTCTGATGAAAGCGAAAATTGATTa                              <  1:361707/43‑1 (MQ=37)                                                                    |                                                              GCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCA  >  minE/2545934‑2546062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑