Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,605,853	T→C	V14V (GTT→GTC)	yhiO →	predicted universal stress (ethanol tolerance) protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,605,853	0	T	C	100.0%	275.9 / NA	74	V14V (GTT→GTC)	yhiO	predicted universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/26);  total (48/26)

CGCGGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAAC  >  minE/2605786‑2605916                                                                    |                                                                cgcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTg                                                               >  1:998608/1‑70 (MQ=255) cgcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTg                                                               >  1:233721/1‑70 (MQ=255) cgcgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTg                                                               >  1:1927451/1‑70 (MQ=255)   cgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCAt                                                            >  1:1129754/1‑71 (MQ=255)   cgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCAt                                                            <  1:1297168/71‑1 (MQ=255)   cgGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCAt                                                            >  1:1616925/1‑71 (MQ=255)      gCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGt                                                         <  1:2151770/71‑1 (MQ=255)        aGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGtt                                                        >  1:2105948/1‑70 (MQ=255)        aGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGtt                                                        >  1:2852269/1‑70 (MQ=255)        aGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGtt                                                        >  1:147570/1‑70 (MQ=255)        aGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGt                                                         >  1:2958437/1‑69 (MQ=255)          gTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAAc                                                     <  1:1673136/71‑1 (MQ=255)           tCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACa                                                    <  1:2890599/71‑1 (MQ=255)            cGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACa                                                    >  1:2269152/1‑70 (MQ=255)            cGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACa                                                    >  1:2444005/1‑70 (MQ=255)             gCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATg                                                  <  1:3169860/71‑1 (MQ=255)                  ggaggaGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGgcgc                                              >  1:3012243/1‑70 (MQ=255)                      gaggTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATgg                                                 >  1:1957193/1‑63 (MQ=255)                        ggTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGgc                                                >  1:1608627/1‑62 (MQ=255)                        ggTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTAttt                                       >  1:363361/1‑71 (MQ=255)                         gTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTtc                                      >  1:355114/1‑71 (MQ=255)                           aTGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTtct                                     >  1:1064187/1‑70 (MQ=255)                              aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCtcat                                  >  1:525644/1‑70 (MQ=255)                              aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCtcat                                  >  1:65268/1‑70 (MQ=255)                              aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCtcat                                  <  1:3139035/70‑1 (MQ=255)                              aTAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCtcat                                  >  1:2657780/1‑70 (MQ=255)                               tAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCtcatca                                >  1:894666/1‑71 (MQ=255)                               tAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCtcatca                                >  1:2371582/1‑71 (MQ=255)                                 aGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCAc                               >  1:1482013/1‑70 (MQ=255)                                  gCACCGTCGCATTATTTTGGGCTTTATGTGTCCTCTGCAt                                                            >  1:2323113/1‑40 (MQ=255)                                     ccGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgc                          >  1:2013060/1‑71 (MQ=255)                                      cGTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgc                          >  1:80351/1‑70 (MQ=255)                                       gTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcgc                        >  1:1818081/1‑71 (MQ=255)                                       gTCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcgc                        >  1:1814339/1‑71 (MQ=255)                                        tCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcgcg                       >  1:462272/1‑71 (MQ=255)                                        tCGCATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTAcgcgcg                       >  1:3155242/1‑71 (MQ=255)                                           cATTATTTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttg                    <  1:2747006/71‑1 (MQ=255)                                            attattTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt                   <  1:30911/71‑1 (MQ=255)                                             ttattTTGGGCTTTATGTGTCGTCTGCATTGTTaa                                                      <  1:2161744/35‑1 (MQ=255)                                             ttattTTGGGCTTTATGTGTCGTCTGCATTGTTaa                                                      <  1:2472947/35‑1 (MQ=255)                                             ttattTTGGGCTTTATGTGTCGTCTGCATTGTTaa                                                      <  1:1060518/35‑1 (MQ=255)                                             ttattTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt                   >  1:1583332/1‑70 (MQ=255)                                             ttattTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt                   >  1:938623/1‑70 (MQ=255)                                             ttattTTGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCgttgt                   >  1:299885/1‑70 (MQ=255)                                                ttttGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGt               <  1:1156477/71‑1 (MQ=255)                                                   tGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTgg             <  1:1048670/70‑1 (MQ=255)                                                   tGGGCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTgg             <  1:874717/70‑1 (MQ=255)                                                    gggCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGt            <  1:3146782/70‑1 (MQ=255)                                                     ggCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTAc          >  1:2784198/1‑71 (MQ=255)                                                      gCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACt         <  1:500428/71‑1 (MQ=255)                                                      gCTTTATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACt         <  1:883575/71‑1 (MQ=255)                                                         ttATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCg      >  1:2984171/1‑71 (MQ=255)                                                         ttATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCg      >  1:3107744/1‑71 (MQ=255)                                                         ttATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCg      >  1:1353610/1‑71 (MQ=255)                                                         ttATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCg      >  1:1357252/1‑71 (MQ=255)                                                          tATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTtctc                                    <  1:3167293/40‑1 (MQ=255)                                                          tATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTtctc                                    <  1:2121320/40‑1 (MQ=255)                                                          tATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTtctc                                    <  1:874804/40‑1 (MQ=255)                                                          tATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTtctc                                    <  1:2485680/40‑1 (MQ=255)                                                          tATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCg      >  1:2301662/1‑70 (MQ=255)                                                          tATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCg      >  1:499409/1‑70 (MQ=255)                                                          tATGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCg      >  1:1772652/1‑70 (MQ=255)                                                           aTGTGTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGt     <  1:512409/70‑1 (MQ=255)                                                            tgtgTCGTCTGCATTGTTAACATGGCGCGCTATTtctc                                    <  1:2516110/38‑1 (MQ=255)                                                            tgtgTCGTCTGCATTGTTAACATGGCGCGCTATTtctc                                    <  1:1795365/38‑1 (MQ=255)                                                            tgtgTCGTCTGCATTGTTAACATGGCGCGCTATTTCtcat                                  >  1:1011547/1‑40 (MQ=255)                                                            tgtgTCGTCTGCATTGTTAACATGGCGCGCTATTTCtcat                                  >  1:1882126/1‑40 (MQ=255)                                                            tgtgTCGTCTGCATTGTTAACATGGCGCGCTATTTCtcat                                  >  1:2453638/1‑40 (MQ=255)                                                            tgtgTCGTCTGCATTGTTAACATGGCGCGCTATTTCtcat                                  >  1:3166073/1‑40 (MQ=255)                                                            tgtgTCGTCTGCATTGTTAACATGGCGCGCTATTTCtca                                   >  1:3159603/1‑39 (MQ=255)                                                            tgtgTCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTaa   <  1:2914451/71‑1 (MQ=255)                                                             gtgtCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAAc  >  1:1274893/1‑71 (MQ=255)                                                              tgtCGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAAc  <  1:2308773/70‑1 (MQ=255)                                                                 cGTCTGCATTGTTAACATGGCGCGCTATTTCTCATCACTa                             >  1:676787/1‑40 (MQ=255)                                                                    |                                                                CGCGGGCAGGTCGCCGGGGAGGAGGTATGATAAGCACCGTCGCATTATTTTGGGCTTTATGTGTCGTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAAC  >  minE/2605786‑2605916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑