Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,625,149	G→A	R16H (CGT→CAT)	ftsX →	predicted transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,625,149	0	G	A	100.0%	229.5 / NA	68	R16H (CGT→CAT)	ftsX	predicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/40);  total (28/40)

GCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCAAA  >  minE/2625086‑2625209                                                                |                                                             gCATGGAGGCGTGGGCCATGAATAAGCGCTATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTg                                                         <  1:1639821/69‑1 (MQ=255) gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTg                                                         <  1:2184091/69‑1 (MQ=255)            tGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTg                                                         <  1:527942/58‑1 (MQ=255)            tGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTaa                                            <  1:464422/71‑1 (MQ=255)             gggCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTaaa                                           >  1:112572/1‑71 (MQ=255)             gggCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTaaa                                           >  1:1314377/1‑71 (MQ=255)                 cATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcgg                                       <  1:480196/71‑1 (MQ=255)                 cATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcgg                                       <  1:538757/71‑1 (MQ=255)                 cATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcgg                                       >  1:2980350/1‑71 (MQ=255)                 cATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcgg                                       >  1:2318388/1‑71 (MQ=255)                 cATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcgg                                       <  1:1924556/71‑1 (MQ=255)                  aTGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATc                                                            <  1:1279399/49‑1 (MQ=255)                  aTGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATc                                                            <  1:1585036/49‑1 (MQ=255)                  aTGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggt                                      >  1:291695/1‑71 (MQ=255)                  aTGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggt                                      >  1:1848387/1‑71 (MQ=255)                    gAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggtcg                                    >  1:2940963/1‑71 (MQ=255)                    gAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggtcg                                    >  1:2805351/1‑71 (MQ=255)                    gAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggtcg                                    >  1:2261065/1‑71 (MQ=255)                    gAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggtcg                                    >  1:95792/1‑71 (MQ=255)                    gAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggtcg                                    >  1:986152/1‑71 (MQ=255)                      aTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCg                                                     >  1:3072750/1‑52 (MQ=255)                      aTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTcggc                                  >  1:1234780/1‑71 (MQ=255)                      aTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTcggc                                  <  1:543006/71‑1 (MQ=255)                       tAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTcggcg                                 <  1:2322588/71‑1 (MQ=255)                         aGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggt                                      >  1:2583506/1‑64 (MQ=255)                          gcgcGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCt                              >  1:1480619/1‑71 (MQ=255)                          gcgcGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCt                              >  1:78127/1‑71 (MQ=255)                           cgcgATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCt                              <  1:2390481/70‑1 (MQ=255)                            gcgATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTc                             >  1:1476164/1‑70 (MQ=255)                            gcgATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTc                             >  1:1596160/1‑70 (MQ=255)                            gcgATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCa                            <  1:1769756/71‑1 (MQ=255)                            gcgATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCa                            <  1:1476109/71‑1 (MQ=255)                                tGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTaaa                                           <  1:173368/52‑1 (MQ=255)                                tGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTaaa                                           <  1:1724407/52‑1 (MQ=255)                                tGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTaaa                                           <  1:1610458/52‑1 (MQ=255)                                tGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTaaa                                           <  1:2202971/52‑1 (MQ=255)                                tGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGc                         >  1:1950038/1‑70 (MQ=255)                                tGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGc                         >  1:1679348/1‑70 (MQ=255)                                  caatcaatCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAc                      <  1:1387041/71‑1 (MQ=255)                                  caatcaatCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAc                      <  1:1590232/71‑1 (MQ=255)                                  caatcaatCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAc                      <  1:3060288/71‑1 (MQ=255)                                  caatcaatCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAc                      <  1:1604164/71‑1 (MQ=255)                                    atcaatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcgg                    <  1:397464/71‑1 (MQ=255)                                    atcaatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcgg                    <  1:2366861/71‑1 (MQ=255)                                    atcaatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcgg                    <  1:2551640/71‑1 (MQ=255)                                    atcaatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcgg                    <  1:1388439/71‑1 (MQ=255)                                       aatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcggcgg                 <  1:1182461/71‑1 (MQ=255)                                       aatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcggcgg                 <  1:1837261/71‑1 (MQ=255)                                       aatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcggcg                  <  1:586539/70‑1 (MQ=255)                                       aatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcggcg                  <  1:3047938/70‑1 (MQ=255)                                       aatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcggcg                  <  1:43029/70‑1 (MQ=255)                                       aatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcggcg                  <  1:1224542/70‑1 (MQ=255)                                          caTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggtcgg                                   >  1:3012174/1‑50 (MQ=255)                                          caTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggtcgg                                   >  1:1602395/1‑50 (MQ=255)                                           atatTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGt             <  1:2808368/71‑1 (MQ=255)                                           atatTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGt             <  1:127962/71‑1 (MQ=255)                                            tatTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTa            <  1:1424175/71‑1 (MQ=255)                                            tatTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTa            <  1:1914338/71‑1 (MQ=255)                                              ttCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCa                            <  1:2563933/53‑1 (MQ=255)                                              ttCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcggcg                  >  1:1516420/1‑63 (MQ=255)                                              ttCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcggcg                  >  1:3035097/1‑63 (MQ=255)                                                cGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACg         >  1:1252294/1‑70 (MQ=255)                                                cGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACg         >  1:2537909/1‑70 (MQ=255)                                                    aGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGt             <  1:837570/62‑1 (MQ=255)                                                    aGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTaa           >  1:1428334/1‑64 (MQ=255)                                                    aGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCAcc     >  1:2380290/1‑70 (MQ=255)                                                      tttGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCaaa  <  1:1657427/71‑1 (MQ=255)                                                      tttGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCaaa  <  1:985578/71‑1 (MQ=255)                                                            gggcaTCTTGAGCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcgg                    <  1:1568946/47‑1 (MQ=255)                                                                |                                                             GCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCAAA  >  minE/2625086‑2625209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑