| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,727,940 | T→C | F59F (TTT→TTC) | kefB → | potassium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,727,940 | 0 | T | C | 100.0% | 69.3 / NA | 20 | F59F (TTT→TTC) | kefB | potassium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/13); total (7/13) | |||||||||||
GGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCA > minE/2727878‑2727998 | gggATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAAc < 1:300315/70‑1 (MQ=255) aTTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCgg < 1:2499099/64‑1 (MQ=255) ttGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTa > 1:3179168/1‑71 (MQ=255) ccGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCt < 1:511997/71‑1 (MQ=255) gTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGa > 1:956826/1‑71 (MQ=255) gcgggggTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTa < 1:2051345/68‑1 (MQ=255) gCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTa < 1:766164/71‑1 (MQ=255) tttATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTAt < 1:178124/52‑1 (MQ=255) tttATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGAt < 1:284679/59‑1 (MQ=255) tttATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGAt < 1:287019/59‑1 (MQ=255) tttATTAGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGAt < 1:2092496/59‑1 (MQ=255) ttattaGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGc > 1:2657005/1‑71 (MQ=255) ttaGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGc > 1:2581703/1‑41 (MQ=255) ttaGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCg > 1:654282/1‑42 (MQ=255) aGCGACGTCGATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTg < 1:2118279/70‑1 (MQ=255) aGCGACGGCGATCAGATCCTCCACTTCTCGGAACTCgg < 1:3118051/38‑1 (MQ=25) gATGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGttt < 1:2294407/48‑1 (MQ=255) tGAGATCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCt > 1:901587/1‑71 (MQ=255) gaTCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTcc > 1:2719102/1‑70 (MQ=255) gaTCCTCCACTTCTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCa < 1:2938412/71‑1 (MQ=255) | GGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCA > minE/2727878‑2727998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |