Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,736,671	T→C	F605F (TTT→TTC)	fusA →	protein chain elongation factor EF‑G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,736,671	0	T	C	100.0%	141.6 / NA	39	F605F (TTT→TTC)	fusA	protein chain elongation factor EF‑G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/26);  total (13/26)

ACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAG  >  minE/2736604‑2736734                                                                    |                                                                aCCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCaa                                                               <  1:546476/70‑1 (MQ=255)    aTGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGaaa                                                           <  1:2090159/71‑1 (MQ=255)        cGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGa                                                       <  1:536203/71‑1 (MQ=255)          ttGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGaaa                                                     <  1:2885207/71‑1 (MQ=255)                cctcTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGtt                                               >  1:2133660/1‑71 (MQ=255)                    tGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCtac                                           >  1:2644415/1‑69 (MQ=255)                    tGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTg                                            >  1:2039481/1‑70 (MQ=255)                    cgAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCtac                                           >  1:2200858/2‑69 (MQ=255)                      aaCTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCtt                                         <  1:853687/71‑1 (MQ=255)                         tGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCaa                                                               <  1:3001750/46‑1 (MQ=255)                          ggCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGaaa                                                     >  1:1288268/1‑55 (MQ=255)                                 aaaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGa                                                       <  1:3028631/46‑1 (MQ=255)                                 aaaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGa                                                       <  1:2992821/46‑1 (MQ=255)                                 aaaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGa                                                       <  1:2316274/46‑1 (MQ=255)                                 aaaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGa                                                       <  1:2311045/46‑1 (MQ=255)                                  aaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg                             <  1:1228349/71‑1 (MQ=255)                                   aCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg                             >  1:1056289/1‑70 (MQ=255)                                   aCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg                             >  1:377369/1‑70 (MQ=255)                                      ggCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAgg                         >  1:3163419/1‑71 (MQ=255)                                           ctTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTg                      <  1:1601461/69‑1 (MQ=255)                                           ctTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTg                      >  1:59572/1‑69 (MQ=255)                                                 tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGaa              <  1:734030/71‑1 (MQ=255)                                                 tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGaa              <  1:490163/71‑1 (MQ=255)                                                 tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa               >  1:2069227/1‑70 (MQ=255)                                                 tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa               <  1:496333/70‑1 (MQ=255)                                                 tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa               <  1:426229/70‑1 (MQ=255)                                                   gCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg                             <  1:1701604/54‑1 (MQ=255)                                                   gCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg                             <  1:1329583/54‑1 (MQ=255)                                                   gCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg                             <  1:404590/54‑1 (MQ=255)                                                    ccTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACt           <  1:2569388/71‑1 (MQ=255)                                                    ccTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACt           <  1:1893577/71‑1 (MQ=255)                                                     cTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTc          <  1:2629935/71‑1 (MQ=255)                                                      tttAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTg                      <  1:520441/58‑1 (MQ=255)                                                      tttAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTg                      <  1:1401852/58‑1 (MQ=255)                                                        tAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCg        >  1:2426934/1‑70 (MQ=255)                                                          aagaagGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGaa     <  1:1465024/71‑1 (MQ=255)                                                           agaagGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCgg       >  1:1927483/1‑68 (MQ=255)                                                            gaagGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGaa     >  1:3050731/1‑69 (MQ=255)                                                              agGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAagag  <  1:1417347/70‑1 (MQ=255)                                                                    |                                                                ACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAG  >  minE/2736604‑2736734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑