Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,811,028	G→A	A573V (GCA→GTA)	uvrA ←	ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,811,028	0	G	A	100.0%	158.6 / NA	49	A573V (GCA→GTA)	uvrA	ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/22);  total (27/22)

GTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGA  >  minE/2810960‑2811093                                                                     |                                                                  gTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACg                                                                 <  1:1173287/71‑1 (MQ=255) gTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCATCCAGCGGACCTTCTACg                                                                 <  1:1046488/71‑1 (MQ=255)    tGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGAc                                                               >  1:2729729/1‑70 (MQ=255)    tGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGAc                                                               >  1:2661548/1‑70 (MQ=255)     gCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCa                                                             >  1:2553523/1‑71 (MQ=255)         cTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCAc                                                            >  1:3081176/1‑68 (MQ=255)         cTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCAc                                                            >  1:1648047/1‑68 (MQ=255)         cTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTc                                                         <  1:1295938/71‑1 (MQ=255)            aTGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAcc                                                      >  1:944528/1‑71 (MQ=255)            aTGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAcc                                                      >  1:1387442/1‑71 (MQ=255)             tGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAccg                                                     >  1:463171/1‑71 (MQ=255)               tACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACtt                                                          >  1:2201052/1‑64 (MQ=255)               tACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACtt                                                          >  1:2122446/1‑64 (MQ=255)               tACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAccgcc                                                   <  1:1171479/71‑1 (MQ=255)               tACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAccgcc                                                   <  1:622864/71‑1 (MQ=255)                aCTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAccgccg                                                  >  1:362942/1‑71 (MQ=255)                aCTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAccg                                                     >  1:2227028/1‑68 (MQ=255)                 cTGCCCGGGCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGt                                                 <  1:857812/71‑1 (MQ=255)                    cccGGTCAACGACTCCGGCACCGCCATATTCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg                                                <  1:3110791/69‑1 (MQ=255)                    cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTg                                                <  1:2791969/69‑1 (MQ=255)                     ccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAac                                             <  1:2746127/71‑1 (MQ=255)                     ccGGTCAACGACTCCGGCACCGCCATAATCGCTTCAAGCGGACCTTCTACGACCACTTCACCGCCGTGaa                                              >  1:1748997/1‑70 (MQ=255)                      cGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAc                                                       <  1:1949780/60‑1 (MQ=255)                        gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACAcc                                          >  1:799224/1‑71 (MQ=255)                        gTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACAcc                                          >  1:1129693/1‑71 (MQ=255)                                 tCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAacac                                           <  1:824289/61‑1 (MQ=255)                                   cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCgg                                 <  1:976354/69‑1 (MQ=255)                                   cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc                               >  1:1410151/1‑71 (MQ=255)                                    ggCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAc                                                       <  1:2182172/46‑1 (MQ=255)                                    ggCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc                               >  1:966918/1‑70 (MQ=255)                                    ggCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGc                               >  1:343178/1‑70 (MQ=255)                                     gCACCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAc                                                       <  1:1057375/45‑1 (MQ=255)                                       aCCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAc                                                       >  1:848333/1‑43 (MQ=255)                                       aCCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAAt                           >  1:341843/1‑71 (MQ=255)                                       aCCGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAAt                           >  1:1193041/1‑71 (MQ=255)                                        ccGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATg                          >  1:2903230/1‑71 (MQ=255)                                        ccGCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATg                          >  1:3060933/1‑71 (MQ=255)                                          gCCATAATCGCTTCCAGCGGACCTTCTACGACCACTTCAccgccg                                                  <  1:109471/45‑1 (MQ=255)                                           ccATAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCg                       <  1:571414/71‑1 (MQ=255)                                             aTAATCGCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGt                                                 >  1:3166903/1‑43 (MQ=255)                                                   gCTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATg               <  1:825237/71‑1 (MQ=255)                                                    cTTCCAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATgg              <  1:1372327/71‑1 (MQ=255)                                                     ttCCAGCGGACCTTCTACGACAACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGt             >  1:1061739/1‑71 (MQ=255)                                                        cAGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg          <  1:865273/71‑1 (MQ=255)                                                        cAGCGGACCTTCTACGACCACTTCACCCCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg          >  1:1551040/1‑71 (MQ=255)                                                         aGCGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAg          >  1:2265680/1‑70 (MQ=255)                                                           cGGACCTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcg        <  1:1343783/70‑1 (MQ=255)                                                                cTTCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGa  <  1:2810213/71‑1 (MQ=255)                                                                 ttCTACGACCACTTCACCGCCGTGAACACCTGCGCCCGGGcc                              <  1:2184341/42‑1 (MQ=255)                                                                     |                                                                  GTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGA  >  minE/2810960‑2811093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑