Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	145,530	T→A	T233S (ACA→TCA)	sfsA ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	145,530	0	T	A	100.0%	126.0 / NA	38	T233S (ACA→TCA)	sfsA	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (16/22);  total (16/22)

TGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTC  >  minE/145463‑145596                                                                    |                                                                   tgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAAc                                                                 >  1:458908/1‑71 (MQ=255) tgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAAc                                                                 >  1:1584786/1‑71 (MQ=255)   aGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCg                                                               >  1:2303730/1‑71 (MQ=255)    ggAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCgg                                                              >  1:825212/1‑71 (MQ=255)       aaaGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAg                                                           >  1:2776662/1‑71 (MQ=255)               tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAAcc                                                                >  1:1265073/1‑58 (MQ=255)               tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGAtttt                                                    >  1:2778387/1‑70 (MQ=255)                      aCGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGc                                            >  1:361478/1‑71 (MQ=255)                       cgcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGcc                                           <  1:1541726/71‑1 (MQ=255)                       cgcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGcc                                           <  1:1950858/71‑1 (MQ=255)                         cgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCAt                                         <  1:890511/71‑1 (MQ=255)                           aCCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGc                                       <  1:1282928/71‑1 (MQ=255)                           aCCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGc                                       <  1:783763/71‑1 (MQ=255)                            ccAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGcc                                      <  1:100331/71‑1 (MQ=255)                                aaTGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCtt                                    >  1:187365/1‑69 (MQ=255)                                 aTGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcag                                 <  1:1950146/71‑1 (MQ=255)                                 aTGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcag                                 >  1:1617099/1‑71 (MQ=255)                                    tAAATTAACCAGTTACTTACTTTACTACAATGAAACCgg                                                              <  1:262412/39‑1 (MQ=255)                                    tAAATTAACCAGTTACTTACTTTACTACAATGAAACCgg                                                              <  1:352509/39‑1 (MQ=255)                                     aaaTTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcagcag                              >  1:2183118/1‑70 (MQ=255)                                            ccAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTcc                      <  1:794867/71‑1 (MQ=255)                                               gTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGaaa                           >  1:511992/1‑63 (MQ=255)                                               gTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGaaa                           >  1:1523623/1‑63 (MQ=255)                                                ttacttacTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCtt                  <  1:481456/71‑1 (MQ=255)                                                       ctttactACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGc           <  1:2806117/71‑1 (MQ=255)                                                         ttactACAATGAAACCGGCAGTGATTTTTTAATAGCCAt                                         <  1:767341/39‑1 (MQ=38)                                                          tactacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGcc          <  1:1125664/69‑1 (MQ=255)                                                          tactacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCa         <  1:1338961/70‑1 (MQ=255)                                                           actacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAAttct                       >  1:1801726/1‑53 (MQ=255)                                                           actacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAAttct                       >  1:873277/1‑53 (MQ=255)                                                             tacAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTcc                      <  1:309140/54‑1 (MQ=255)                                                               cAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGTCAGAAt     <  1:412153/69‑1 (MQ=255)                                                               cAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAttt   <  1:2502644/71‑1 (MQ=255)                                                               cAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt     <  1:304186/69‑1 (MQ=255)                                                               cAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt     <  1:2327107/69‑1 (MQ=255)                                                               cAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt     <  1:199281/69‑1 (MQ=255)                                                                aaTGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTc  >  1:1970617/1‑71 (MQ=255)                                                                 aTGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTc                       <  1:1831104/49‑1 (MQ=255)                                                                    |                                                                   TGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTC  >  minE/145463‑145596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑