Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,892,177	G→A	D278N (GAC→AAC)	ytfQ →	predicted sugar transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,892,177	0	G	A	100.0%	121.4 / NA	37	D278N (GAC→AAC)	ytfQ	predicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/15);  total (22/15)

TGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCC  >  minE/2892118‑2892243                                                            |                                                                   tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTg                                                            >  1:2821010/1‑68 (MQ=255)     tggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGaaa                                                      <  1:603735/70‑1 (MQ=255)         gaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaa                                                 <  1:1399224/71‑1 (MQ=255)           agcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaaaa                                               <  1:36435/71‑1 (MQ=255)           agcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaaa                                                >  1:1159838/1‑70 (MQ=255)           agcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaaa                                                >  1:1979620/1‑70 (MQ=255)              gaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaaaa                                               >  1:1780249/1‑68 (MQ=255)               aaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGa                                           <  1:164235/71‑1 (MQ=255)                aCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGAc                                          <  1:578168/71‑1 (MQ=255)                 cGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACg                                         <  1:1083123/71‑1 (MQ=255)                 cGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACg                                         >  1:2361930/1‑71 (MQ=255)                   ccAGTGTTGAACTGACGCCGAATATTGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACgg                                        >  1:553870/1‑70 (MQ=255)                   ccAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACgg                                        >  1:2715594/1‑70 (MQ=255)                   ccAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACgg                                        >  1:2614359/1‑70 (MQ=255)                    cAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACg                                         <  1:30998/68‑1 (MQ=255)                    cAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACg                                         <  1:1413664/68‑1 (MQ=255)                       tgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATAc                                                   <  1:1118039/55‑1 (MQ=255)                        gtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCAt                                  >  1:2307270/1‑71 (MQ=255)                         ttGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTg                                                            <  1:2527681/44‑1 (MQ=255)                         ttGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTg                                                            <  1:1368175/44‑1 (MQ=255)                         ttGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATg                                 >  1:1126973/1‑71 (MQ=255)                             aCTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCt                              >  1:965392/1‑70 (MQ=255)                             aCTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCt                              >  1:1538707/1‑70 (MQ=255)                                   gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCa                                      >  1:1315438/1‑56 (MQ=255)                                      gAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGa                                           >  1:131449/1‑48 (MQ=255)                                         tatGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACa                                                  <  1:2780424/38‑1 (MQ=255)                                         tatGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACa                                                  <  1:2114735/38‑1 (MQ=255)                                               aGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAcc            >  1:727807/1‑70 (MQ=255)                                               aGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAcc            >  1:2592898/1‑70 (MQ=255)                                                ggTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGt                 >  1:298256/1‑64 (MQ=255)                                                ggTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCaa          <  1:160837/71‑1 (MQ=255)                                                ggTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCa           >  1:1683720/1‑70 (MQ=255)                                                    ccGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTg                     >  1:1743859/1‑56 (MQ=255)                                                     cGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTg                             <  1:2483694/47‑1 (MQ=255)                                                        cTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAc             >  1:1869735/1‑60 (MQ=255)                                                        cTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAccc  >  1:3141511/1‑71 (MQ=255)                                                        cTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAccc  >  1:941329/1‑71 (MQ=255)                                                            |                                                                   TGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCC  >  minE/2892118‑2892243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑