Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,895,458	G→A	A164A (GCG→GCA)	yjfF →	predicted sugar transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,895,458	0	G	A	100.0%	245.0 / NA	73	A164A (GCG→GCA)	yjfF	predicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/39);  total (34/39)

AACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGG  >  minE/2895390‑2895523                                                                     |                                                                  aaCCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAAt                                                                 <  1:1670160/71‑1 (MQ=255)  aCCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATg                                                                <  1:2037515/71‑1 (MQ=255)   ccATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATgg                                                               <  1:503770/71‑1 (MQ=255)   ccATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATgg                                                               <  1:316168/71‑1 (MQ=255)       cccATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGAct                                                           >  1:375902/1‑71 (MQ=255)            ttATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGa                                                      >  1:1770695/1‑71 (MQ=255)             tATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGa                                                      >  1:925040/1‑70 (MQ=255)              aTGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGAt                                                     <  1:2987910/70‑1 (MQ=255)              aTGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGAt                                                     <  1:305647/70‑1 (MQ=255)                  cacGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGtt                                                  >  1:1602412/1‑69 (MQ=255)                  cacGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTgg                                                <  1:94347/71‑1 (MQ=255)                           aaGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGt                                                   >  1:180640/1‑59 (MQ=255)                           aaGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGt                                                   >  1:2155722/1‑59 (MQ=255)                           aaGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggtgg                                          <  1:359155/68‑1 (MQ=255)                           aaGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggtgg                                          <  1:448430/68‑1 (MQ=255)                              ccTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTa                                    <  1:1716217/71‑1 (MQ=255)                              ccTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTa                                    <  1:383329/71‑1 (MQ=255)                               cTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGActtct                                                        <  1:695712/50‑1 (MQ=39)                               cTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGActgct                                                        <  1:3143269/50‑1 (MQ=255)                               cTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGActgct                                                        <  1:1119289/50‑1 (MQ=255)                                 tGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggtggtgg                                       >  1:286258/1‑65 (MQ=255)                                 tGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggtggtgg                                       >  1:2799945/1‑65 (MQ=255)                                  gCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATggg                                                              >  1:1784209/1‑41 (MQ=255)                                  gCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCgg                                >  1:2727069/1‑71 (MQ=255)                                  gCGTGGAAAATCCATGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCgg                                >  1:353385/1‑71 (MQ=255)                                       gAAAATCCCTGGCGGCGGTCGCTTAAGTGCAAGGGGa                                                             >  1:373867/1‑37 (MQ=38)                                        aaaaTCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCg                                              <  1:2429264/51‑1 (MQ=255)                                         aaaTCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTc                         >  1:2457719/1‑71 (MQ=255)                                            tCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCa                              >  1:1607490/1‑63 (MQ=255)                                            tCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTc                      >  1:2877265/1‑71 (MQ=255)                                             cccTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCata                            <  1:693767/64‑1 (MQ=255)                                             cccTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCata                            <  1:2441303/64‑1 (MQ=255)                                             cccTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCata                            <  1:2637039/64‑1 (MQ=255)                                              ccTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATc                                  <  1:2069547/57‑1 (MQ=255)                                              ccTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTcgc                    <  1:3006811/71‑1 (MQ=255)                                              ccTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTcgc                    <  1:2842960/71‑1 (MQ=255)                                              ccTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTcg                     <  1:1128855/70‑1 (MQ=255)                                              ccTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTcg                     <  1:436311/70‑1 (MQ=255)                                                  gcggcgGGCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCa                 <  1:680834/70‑1 (MQ=255)                                                    ggcggTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggtggtgg                                       <  1:1826073/46‑1 (MQ=255)                                                    ggcggTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggtggtgg                                       <  1:1909752/46‑1 (MQ=255)                                                     gcggTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGt             <  1:539650/71‑1 (MQ=255)                                                     gcggTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGt             <  1:269359/71‑1 (MQ=255)                                                      cggTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTa            <  1:1544783/71‑1 (MQ=255)                                                       ggTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTcgcg                   >  1:1424272/1‑63 (MQ=255)                                                       ggTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTcgcg                   >  1:580768/1‑63 (MQ=255)                                                        gTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCg                                 <  1:472911/48‑1 (MQ=255)                                                        gTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCg                                 <  1:2838152/48‑1 (MQ=255)                                                        gTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCata                            <  1:3068576/53‑1 (MQ=255)                                                        gTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTa            >  1:3173321/1‑69 (MQ=255)                                                          cGCTTAGGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGc                               >  1:411533/1‑48 (MQ=39)                                                           gCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTTGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGt       <  1:759230/71‑1 (MQ=255)                                                           gCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGt       >  1:1779458/1‑71 (MQ=255)                                                           gCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGt       >  1:1670719/1‑71 (MQ=255)                                                            cTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGt             <  1:3191922/64‑1 (MQ=255)                                                             ttAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGttt     >  1:1223666/1‑71 (MQ=255)                                                             ttAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGttt     >  1:221959/1‑71 (MQ=255)                                                              tAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTc                         >  1:348754/1‑50 (MQ=255)                                                              tAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTc                         >  1:1750896/1‑50 (MQ=255)                                                              tAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGtttt    >  1:2960043/1‑71 (MQ=255)                                                              tAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGtttt    >  1:532627/1‑71 (MQ=255)                                                               aaGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGtttt    <  1:1855343/70‑1 (MQ=255)                                                               aaGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGttt     <  1:197074/69‑1 (MQ=255)                                                               aaGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTg   >  1:1487779/1‑71 (MQ=255)                                                               aaGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTg   >  1:1234454/1‑71 (MQ=255)                                                               aaGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTg   >  1:2556807/1‑71 (MQ=255)                                                               aaGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTg   >  1:2670891/1‑71 (MQ=255)                                                               aaGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTg   >  1:1766758/1‑71 (MQ=255)                                                                aGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGtttt    <  1:2561229/69‑1 (MQ=255)                                                                aGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTgg  >  1:1165085/1‑71 (MQ=255)                                                                aGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTgg  <  1:3038257/71‑1 (MQ=255)                                                                aGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTgg  >  1:320830/1‑71 (MQ=255)                                                                aGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTg   <  1:1447592/70‑1 (MQ=255)                                                                     |                                                                  AACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGG  >  minE/2895390‑2895523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑