Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,973,690	A→T	K29I (AAA→ATA)	nadR →	bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,973,690	0	A	T	100.0%	179.6 / NA	54	K29I (AAA→ATA)	nadR	bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (17/37);  total (17/37)

CTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTG  >  minE/2973623‑2973757                                                                    |                                                                    cTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAgg                                                                  <  1:1207019/71‑1 (MQ=255) cTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAgg                                                                  <  1:1347212/71‑1 (MQ=255) cTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAgg                                                                  <  1:1491478/71‑1 (MQ=255)    aaaaCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTa                                                               >  1:2919162/1‑71 (MQ=255)    aaaaCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTa                                                               >  1:3119705/1‑71 (MQ=255)     aaaCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTAt                                                              >  1:105248/1‑71 (MQ=255)       aCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTAttt                                                            <  1:225268/71‑1 (MQ=255)           ccATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTaa                                                          <  1:1385006/69‑1 (MQ=255)           ccATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTaa                                                          <  1:194387/69‑1 (MQ=255)           ccATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGc                                                        <  1:324304/71‑1 (MQ=255)             aTCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGc                                                        >  1:616749/1‑69 (MQ=255)                  gCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTAc                                                 <  1:1155189/71‑1 (MQ=255)                      cAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGaa                                             <  1:2002115/71‑1 (MQ=255)                      cAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAAGGTATTTAAGCCAGTTACTGaa                                             <  1:258975/71‑1 (MQ=255)                       aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTa                                                           <  1:1567058/56‑1 (MQ=255)                       aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTa                                                           <  1:2954155/56‑1 (MQ=255)                       aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTa                                                           <  1:2437657/56‑1 (MQ=255)                       aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTa                                                           <  1:1374112/56‑1 (MQ=255)                       aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTa                                                           <  1:1247936/56‑1 (MQ=255)                       aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTg                                               <  1:796276/68‑1 (MQ=255)                         ggCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGaa                                             >  1:1499686/1‑68 (MQ=255)                             gCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaa                                       >  1:680422/1‑70 (MQ=255)                             gCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaa                                       >  1:2055535/1‑70 (MQ=255)                             gCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaa                                       >  1:2524162/1‑70 (MQ=255)                              cACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa                                     >  1:2048855/1‑71 (MQ=255)                              cACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa                                     >  1:2806239/1‑71 (MQ=255)                                cGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATc                                   <  1:2375025/71‑1 (MQ=255)                                 gCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCa                                  <  1:796349/71‑1 (MQ=255)                                  cTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGa                                              <  1:2490229/58‑1 (MQ=255)                                  cTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa                                 <  1:584863/71‑1 (MQ=255)                                  cTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa                                 <  1:2778824/71‑1 (MQ=255)                                  cTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa                                 <  1:1358022/71‑1 (MQ=255)                                  cTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCa                                  >  1:989718/1‑70 (MQ=255)                                   tACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa                                 >  1:2787984/1‑70 (MQ=255)                                   tACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa                                 >  1:1660525/1‑70 (MQ=255)                                     cagcagGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaaa                                <  1:2519156/69‑1 (MQ=255)                                     cagcagGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaaa                                <  1:1507528/69‑1 (MQ=255)                                       gcagGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcc                            <  1:2115686/71‑1 (MQ=255)                                       gcagGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcc                            <  1:260709/71‑1 (MQ=255)                                           gTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaa                                       <  1:1705998/56‑1 (MQ=255)                                           gTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaa                                       <  1:1874375/56‑1 (MQ=255)                                             aGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa                                     >  1:1633471/1‑56 (MQ=255)                                             aGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaa                                      >  1:707186/1‑55 (MQ=255)                                                 gATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcgcgc                   >  1:2780825/1‑70 (MQ=255)                                                  aTGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaa                 <  1:972198/71‑1 (MQ=255)                                                     ccAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAg              <  1:101924/71‑1 (MQ=255)                                                        gCGGTATGACCATATGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg           <  1:3070855/71‑1 (MQ=255)                                                        gCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg           <  1:229726/71‑1 (MQ=255)                                                         cGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAATAGCTg           <  1:1816837/70‑1 (MQ=255)                                                         cGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTgg          <  1:866293/71‑1 (MQ=255)                                                                aCCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGt    <  1:1000035/70‑1 (MQ=255)                                                                aCCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGt    <  1:1519547/70‑1 (MQ=255)                                                                 ccATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTgg          <  1:623815/63‑1 (MQ=255)                                                                 ccATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTg  >  1:427897/1‑71 (MQ=255)                                                                    |                                                                    CTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTG  >  minE/2973623‑2973757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑