Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	204,556	A→G	intergenic (+156/‑28)	alaV → / → rrlH	tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	204,556	0	A	G	100.0%	136.4 / NA	45	intergenic (+156/‑28)	alaV/rrlH	tRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/20);  total (25/20)

TGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTG  >  minE/204496‑204618                                                             |                                                               tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa                                                       >  1:1543062/1‑70 (MQ=255)      cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc                                                 >  1:878041/1‑71 (MQ=255)            acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg                                           <  1:2497525/71‑1 (MQ=255)            acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg                                           <  1:168573/71‑1 (MQ=255)             caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt                                          >  1:2114364/1‑71 (MQ=255)             caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt                                          >  1:2110613/1‑71 (MQ=255)                   aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                                    >  1:2678241/1‑71 (MQ=21)                         ttCGTGAGTCTATCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAg                              >  1:1997576/1‑71 (MQ=255)                               aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                         >  1:1856108/1‑70 (MQ=25)                               aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTa                        <  1:737198/71‑1 (MQ=21)                                 tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt                                               <  1:1646016/46‑1 (MQ=255)                                  ctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt                                               <  1:400216/45‑1 (MQ=255)                                   tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc                                              >  1:1510362/1‑45 (MQ=255)                                   tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg                                             >  1:9759/1‑46 (MQ=255)                                   tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg                                             >  1:1865890/1‑46 (MQ=255)                                   tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg                                             >  1:1478210/1‑46 (MQ=255)                                   tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCg                                             >  1:1540456/1‑46 (MQ=255)                                     tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                    <  1:1043667/69‑1 (MQ=25)                                     tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                    <  1:183691/69‑1 (MQ=25)                                     tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                    <  1:1298189/69‑1 (MQ=25)                                     tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                    <  1:1867132/69‑1 (MQ=25)                                     tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                  >  1:527357/1‑71 (MQ=21)                                     tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                  >  1:3190406/1‑71 (MQ=21)                                     tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                  >  1:3135840/1‑71 (MQ=21)                                     tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                  >  1:2899840/1‑71 (MQ=21)                                        aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                             <  1:3062503/57‑1 (MQ=255)                                        aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac               <  1:1624725/71‑1 (MQ=21)                                         aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg              >  1:2996729/1‑71 (MQ=21)                                         aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg              >  1:964334/1‑71 (MQ=21)                                         aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg              >  1:171351/1‑71 (MQ=21)                                         aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg              >  1:2399487/1‑71 (MQ=21)                                          ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg             >  1:2606230/1‑71 (MQ=21)                                          ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg             >  1:2288328/1‑71 (MQ=21)                                          ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg             >  1:1918242/1‑71 (MQ=21)                                          ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACgg             <  1:782777/71‑1 (MQ=21)                                           tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa                           >  1:1689094/1‑56 (MQ=255)                                             tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg           <  1:2514642/70‑1 (MQ=25)                                                    acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt   <  1:1950018/71‑1 (MQ=21)                                                    acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt   <  1:2115042/71‑1 (MQ=21)                                                    acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt   <  1:1644097/71‑1 (MQ=21)                                                    acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt   <  1:662383/71‑1 (MQ=21)                                                    acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt   <  1:1917953/71‑1 (MQ=21)                                                     cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc    <  1:49189/69‑1 (MQ=25)                                                     cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc    <  1:2006182/69‑1 (MQ=25)                                                       atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTg  >  1:1404785/1‑69 (MQ=25)                                                             |                                                               TGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTG  >  minE/204496‑204618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑