| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 21,286 | (CTG)2→1 | coding (1218‑1220/2817 nt) | ileS → | isoleucyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 21,281 | 0 | T | . | 97.6% | 351.8 / 1.2 | 82 | coding (1213/2817 nt) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base . (0/80); total (0/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | minE | 21,282 | 0 | G | . | 98.8% | 355.7 / ‑2.0 | 82 | coding (1214/2817 nt) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (0/81); total (0/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
| * | minE | 21,283 | 0 | C | . | 98.8% | 355.5 / ‑0.7 | 82 | coding (1215/2817 nt) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (0/81); total (0/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
GCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGT > minE/21268‑21332 ||| gcaCAGCTATCCGT‑‑T‑CTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:217368/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCCGTGGt < 1:928527/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTTGt < 1:141275/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:538448/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:692404/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:68569/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:659010/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:647981/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:638219/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:623908/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:591828/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:582239/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:56356/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:703991/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:534899/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:533764/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:529739/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:527768/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:1016637/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:500976/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:483098/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:8645/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:998800/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:982909/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:977172/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:94956/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:934749/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:912156/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:887942/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:869924/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:696851/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:852908/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:832399/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:802372/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:785798/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:780665/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:705544/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:513823/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:190652/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:312419/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:304571/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:303374/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:281105/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:273999/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:256048/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:235907/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:234688/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:210974/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:481310/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:189746/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:184808/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:177126/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:17125/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:139027/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:128357/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:120711/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:113241/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:455362/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:443100/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:436290/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:435124/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:428422/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:412270/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:331552/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:375902/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:447751/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:46714/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:482575/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:366152/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:333362/62‑1 (MQ=255)gcaCAGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATATTCCGCGCGACGCCGCAGTGGt < 1:748682/62‑1 (MQ=255) cacaGCTATCCGTGCTG‑T‑‑CGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:370014/61‑1 (MQ=255) cacaGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:395542/61‑1 (MQ=255) cacaGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:985977/61‑1 (MQ=255) cacaGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:1033096/61‑1 (MQ=255) cacaGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:143138/61‑1 (MQ=255) cacaGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:615844/61‑1 (MQ=255) cacaGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:778445/61‑1 (MQ=255) cacaGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:760657/61‑1 (MQ=255) cacaGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:620011/61‑1 (MQ=255) cacaGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGt < 1:687767/61‑1 (MQ=255) cacaGCTATCCG‑‑‑TGCTGGCGTCACAAAACGCCCATCATCTTCCGCGCGACGCCGCAGTGGt < 1:619819/61‑1 (MQ=255) ||| GCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGT > minE/21268‑21332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |