Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,764,743	Δ1 bp	coding (2117/2574 nt)	clpB ←	protein disaggregation chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,764,743	0	G	.	100.0%	125.1 / NA	29	coding (2117/2574 nt)	clpB	protein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (0/29);  total (0/29)

AAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATG  >  minE/1764734‑1764796          |                                                      aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:133508/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:945958/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:870468/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:862120/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:855622/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:755427/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:671103/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:665/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:655664/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:641699/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:626012/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:558520/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:27468/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:120563/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:134157/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:173612/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:177090/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:192060/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:443928/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:312158/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:336708/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:401497/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:413481/62‑1 (MQ=255) aaGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:428360/62‑1 (MQ=255) aaGGCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:81110/62‑1 (MQ=255)  aGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:540900/61‑1 (MQ=255)  aGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:888709/61‑1 (MQ=255)  aGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:916349/61‑1 (MQ=255)  aGTCGACC‑TTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATg  <  1:474644/61‑1 (MQ=255)          |                                                      AAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATG  >  minE/1764734‑1764796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑