Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,293,884	+A	coding (342/951 nt)	cbl ←	DNA‑binding transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,293,880	1	.	A	100.0%	25.9 / NA	9	R116C (CGC→TGC)	cbl	DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (9/0);  total (9/0)

CCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCG‑AAAAGCTTT  >  minE/1293829‑1293889                                                     |          ccagtgGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAAGCttt  >  1:929743/4‑62 (MQ=255) ccTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAAGCttt  >  1:1004090/1‑62 (MQ=255) ccTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAAGCttt  >  1:339811/1‑62 (MQ=255) ccTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAAGCttt  >  1:496529/1‑62 (MQ=255) ccTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAAGCttt  >  1:512508/1‑62 (MQ=255) ccTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAAGCttt  >  1:56064/1‑62 (MQ=255) ccTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAAGCttt  >  1:597713/1‑62 (MQ=255) ccTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAAGCttt  >  1:775897/1‑62 (MQ=255) ccTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAAGCttt  >  1:829798/1‑62 (MQ=255)                                                     |          CCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCG‑AAAAGCTTT  >  minE/1293829‑1293889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑