| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,394,832 | 0 | G | A | 58.0% | 41.7 / 114.1 | 88 | A32A (GCC→GCT) | fruB | fused fructose‑specific PTS enzyme IIA component and HPr component |
| Reads supporting (aligned to +/- strand): ref base G (37/0); new base A (51/0); total (88/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGG > minE/1394799‑1394884 | cATGCCATTGACGTAGCCTTCTGTTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:192907/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGAcc > 1:945297/1‑58 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCtt > 1:702611/1‑59 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCt > 1:506295/1‑59 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:661126/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:916319/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:497688/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:510416/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:537170/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:540024/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:908968/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:907112/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:604321/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:612621/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:636712/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:639622/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:641997/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:1022964/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:671619/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:704476/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:705424/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:717943/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:885686/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:720216/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:883758/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:860359/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:849464/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:847211/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:795302/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:237013/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:140185/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:146542/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:151572/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:136798/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:184790/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:199253/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:224994/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:410001/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:134094/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:242376/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:983940/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:251836/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:258140/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:26267/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:94339/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:377493/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCAGCGACCTg > 1:385473/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGCACCGACCTg > 1:970191/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACCAGCCTGCACCAGCGCCGAAGCGACCTg > 1:810192/1‑60 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTACAAGCCTGCACCAGCGCCGCAGCGACCt > 1:262665/1‑59 (MQ=255)cATGCCATTGACGTAGCCTTCTGCTACATTAACAGCCTg > 1:653113/1‑39 (MQ=38) tACATTACCGGCCTGCACCAGCGCCGCCGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:81165/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCg > 1:89387/1‑44 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCtcttc > 1:611357/1‑51 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:898876/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:8503/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:993535/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:970079/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:966301/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:955216/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:903551/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:936755/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:923018/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:913281/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:414823/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:1036399/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:123474/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:148110/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:154674/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:226196/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:227375/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:26739/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:373550/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:396283/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:410363/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:779892/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:552100/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:617512/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:656677/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:719560/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:732540/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:750024/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:754661/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:755759/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCgg > 1:768260/1‑62 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCg > 1:676342/1‑61 (MQ=255) tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCg > 1:241288/1‑61 (MQ=255) tACATTACCGGCCTGCACCAGAGCCGCAGCGACCTGGCGAATCGCctct > 1:597339/1‑49 (MQ=255) | CATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGG > minE/1394799‑1394884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |