| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,695,001 | 1 | . | C | 48.9% | 253.0 / inf | 131 | coding (55/561 nt) | nudE | ADP‑ribose diphosphatase |
| Reads supporting (aligned to +/- strand): ref base . (1/66); new base C (64/0); total (65/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.55e-37 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 132 total aligned reads displayed.
TTACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCGAG > minE/2694959‑2695021 | ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCGTTC‑CCCGACTGTTTACCGt > 1:895418/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTAcc > 1:555459/1‑58 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTAcag > 1:67858/1‑57 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:557927/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:813315/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:387594/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:395613/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:410430/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:421668/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:798796/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:457498/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:794230/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:773043/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:50517/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:528871/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:531387/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:385095/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:767494/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:757030/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:597493/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:607768/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:619179/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:624338/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:651015/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:658430/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:660671/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:704155/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:683748/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:697870/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:697903/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:838003/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:163846/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:912802/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:9228/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:152946/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:884800/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:875363/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:871356/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:109036/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:291881/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:1036920/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:101626/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:1000594/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:832195/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:319881/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:822528/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:343833/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:817850/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCg > 1:819116/1‑59 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCGt > 1:1001014/1‑60 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTTCCCCGACTGTTTACCGTc > 1:246163/1‑61 (MQ=255)ttACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑c > 1:810252/1‑44 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:912258/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:902863/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:901232/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:740234/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:888972/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:737828/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:942223/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:965306/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:790902/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:971874/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:837996/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:813299/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:993975/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:307007/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:1015972/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:106701/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:131122/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:150523/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:161687/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:171086/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:17168/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:184425/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:209382/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:228486/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:260312/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:269335/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:293816/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:683232/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:31315/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:372488/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:594211/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:567841/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:498650/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:49484/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:457141/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:442819/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:43329/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:384214/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:375130/62‑1 (MQ=255) tACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:332204/62‑1 (MQ=255) aCAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:904675/61‑1 (MQ=255) aCAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:159471/61‑1 (MQ=255) aCAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:741478/61‑1 (MQ=255) aCAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:615673/61‑1 (MQ=255) cAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:934042/60‑1 (MQ=255) cAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:641978/60‑1 (MQ=255) ccaTTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:466290/50‑1 (MQ=255) tGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCgag < 1:255575/44‑1 (MQ=255) | TTACAAAAACCCACCATTCTGAATGTTGAAACTGTAGCCCGTT‑CCCGACTGTTTACCGTCGAG > minE/2694959‑2695021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |