Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,967,244	G→A	D462D (GAC→GAT)	tktA ←	transketolase 1, thiamin‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,967,244	0	G	A	100.0%	14.2 / NA	10	D462D (GAC→GAT)	tktA	transketolase 1, thiamin‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/10);  total (0/10)

GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT  >  minE/1967193‑1967254                                                    |           gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga  <  1:1003303/62‑2 (MQ=12) gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga  <  1:1030947/62‑2 (MQ=12) gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga  <  1:33402/62‑2 (MQ=12) gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga  <  1:480844/62‑2 (MQ=12) gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga  <  1:620481/62‑2 (MQ=12) gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga  <  1:661560/62‑2 (MQ=12) gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga  <  1:894375/62‑2 (MQ=12) gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga  <  1:924550/62‑2 (MQ=12) gACGTGCTCGATCGACTGGTGGGTCGGGCCGTCTTCGCACAGGCCGATCGAATCGTGGgtga  <  1:948588/62‑2 (MQ=255)                         cGGGCCGTCTTCGCCCAGGCCGATCGAATCGTGGgtga  <  1:859212/38‑2 (MQ=17)                                                    |           GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT  >  minE/1967193‑1967254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑