Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,111,795	T→C	C147R (TGC→CGC)	agaI →	galactosamine‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,111,795	0	T	C	100.0%	24.9 / NA	8	C147R (TGC→CGC)	agaI	galactosamine‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/8);  total (0/8)

AACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCA  >  minE/2111762‑2111823                                  |                             aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa  <  1:122925/62‑1 (MQ=255) aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa  <  1:272610/62‑1 (MQ=255) aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa  <  1:44227/62‑1 (MQ=255) aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa  <  1:538380/62‑1 (MQ=255) aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa  <  1:590260/62‑1 (MQ=255) aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa  <  1:852461/62‑1 (MQ=255) aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGTCa  <  1:920292/62‑1 (MQ=255) aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTACGCGTTCTCGGATTGGGGAAAAACGGGCa  <  1:696942/62‑1 (MQ=255)                                  |                             AACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCA  >  minE/2111762‑2111823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑