| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,236,063 | G→A | V403V (GTG→GTA) | purH → | fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,236,063 | 0 | G | A | 100.0% | 25.7 / NA | 9 | V403V (GTG→GTA) | purH | fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/9); total (0/9) | |||||||||||
CGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAT > minE/2236042‑2236102 | cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:1005514/61‑1 (MQ=255)cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:1022810/61‑1 (MQ=255)cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:104739/61‑1 (MQ=255)cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:396966/61‑1 (MQ=255)cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:502255/61‑1 (MQ=255)cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:512654/61‑1 (MQ=255)cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:597139/61‑1 (MQ=255)cGGTGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGGGAt < 1:898561/61‑1 (MQ=255) tGCGGAAGAACTGCGCGTAGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAt < 1:971902/58‑1 (MQ=255) | CGGTGCGGAAGAACTGCGCGTGGTGACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGAT > minE/2236042‑2236102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |