Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,906,397	C→A	G390C (GGC→TGC)	treB ←	fused trehalose(maltose)‑specific enzyme IIBC component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,906,397	0	C	A	100.0%	189.7 / NA	55	G390C (GGC→TGC)	treB	fused trehalose(maltose)‑specific enzyme IIBC component of PTS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/55);  total (0/55)

CAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAG  >  minE/2906346‑2906408                                                    |            cagaACCAATCATCGCGCACAGCATC‑GGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:961207/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:76900/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:510334/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:1003857/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:539421/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:572118/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:603935/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:610854/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:614267/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:61842/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:624091/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:646654/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:715797/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:742244/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:529743/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:776481/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:780738/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:790730/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:838507/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:876379/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:887112/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:925195/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:964937/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:983177/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:991010/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:268387/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:1009876/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:114206/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:136789/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:145184/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:155472/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:156388/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:162263/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:163068/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:186966/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:309207/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:320922/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:453335/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:449341/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:447474/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:44330/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:439713/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:356942/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:341936/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:337918/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGAAGTACATTGCAg  <  1:184894/62‑1 (MQ=255)  agaACCAATCATCGCGCACAGCATCGGGAAGCGAAATTTCAGGTTGATGCAGTACATTGCAg  <  1:975272/62‑1 (MQ=255)   gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:869882/61‑1 (MQ=255)   gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:435659/61‑1 (MQ=255)   gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:940297/61‑1 (MQ=255)   gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:468269/61‑1 (MQ=255)   gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:28822/61‑1 (MQ=255)   gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:721514/61‑1 (MQ=255)   gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:663215/61‑1 (MQ=255)               gcgcACAGCATCGGGAAGCGATATTTCAGGTTGATGCAGTACATTGCAg  <  1:192279/49‑1 (MQ=255)                                                    |            CAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAG  >  minE/2906346‑2906408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑