Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	687,999	G→T	T40N (ACT→AAT)	yccA ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	687,999	0	G	T	100.0%	25.6 / NA	9	T40N (ACT→AAT)	yccA	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/0);  total (9/0)

ATACCCACCAGCGTCAGAATCAGACCCGGAGATGGCAGCATCAGCACCGTACTGGCAGTTGC  >  minE/687942‑688003                                                          |     atacCCACCAGCGTCAGAATCAGACCCGGAGATGGCAGCATCAGCACCGTACTGGCATTTGc  >  1:1036365/1‑62 (MQ=255) atacCCACCAGCGTCAGAATCAGACCCGGAGATGGCAGCATCAGCACCGTACTGGCATTTGc  >  1:184054/1‑62 (MQ=255) atacCCACCAGCGTCAGAATCAGACCCGGAGATGGCAGCATCAGCACCGTACTGGCATTTGc  >  1:236551/1‑62 (MQ=255) atacCCACCAGCGTCAGAATCAGACCCGGAGATGGCAGCATCAGCACCGTACTGGCATTTGc  >  1:27615/1‑62 (MQ=255) atacCCACCAGCGTCAGAATCAGACCCGGAGATGGCAGCATCAGCACCGTACTGGCATTTGc  >  1:533479/1‑62 (MQ=255) atacCCACCAGCGTCAGAATCAGACCCGGAGATGGCAGCATCAGCACCGTACTGGCATTTGc  >  1:536604/1‑62 (MQ=255) atacCCACCAGCGTCAGAATCAGACCCGGAGATGGCAGCATCAGCACCGTACTGGCATTTGc  >  1:539580/1‑62 (MQ=255) atacCCACCAGCGTCAGAATCAGACCCGGAGATGGCAGCATCAGCACCGTACTGGCATTTGc  >  1:615551/1‑62 (MQ=255) atacCCACCAGCGTCAGAATCAGACCCGGAGATGGCAGCATCAGCACCGTACTGGCATTTGc  >  1:65013/1‑62 (MQ=255)                                                          |     ATACCCACCAGCGTCAGAATCAGACCCGGAGATGGCAGCATCAGCACCGTACTGGCAGTTGC  >  minE/687942‑688003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑