Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,202,910	A→G	T190A (ACG→GCG)	yoaD →	predicted phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,202,910	0	A	G	100.0%	109.9 / NA	30	T190A (ACG→GCG)	yoaD	predicted phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/0);  total (30/0)

CCACAACTGCCGCAAATCAGTAGCGCCAGCCTGACGGTGGACAAACGGCATTTGCTCTATGGTAATGGGC  >  minE/1202877‑1202946                                  |                                     ccACAACTGCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATg           >  1:435735/1‑61 (MQ=255) ccACAACTGCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATg           >  1:1010077/1‑61 (MQ=255) ccACAACTGCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATg           >  1:183825/1‑61 (MQ=255) ccACAACTGCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATg           >  1:375505/1‑61 (MQ=255) ccACAACTGCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATg           >  1:288777/1‑61 (MQ=255) ccACAACTGCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATg           >  1:668707/1‑61 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:792097/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:525382/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:564501/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:63531/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:719807/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:740592/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:417481/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:795706/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:85676/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:867219/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:948017/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:958827/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:435004/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:100640/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:372089/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:370004/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:352913/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:294007/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:289334/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:257910/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:184845/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGCGGTGGACAAACGGCATTTGCTCTATGGTAATGGGc  >  1:138471/1‑62 (MQ=255)         gCCGCAAATCAGTAGCGCCAGCCTGGAGGTGGACAAACGGCAtt                    >  1:1020292/1‑44 (MQ=38)         gCCGCAAATCAGTAGCGCCAGCCCGGCGGTGGACAAACCGCATTTGCTCTATGGTAATGGGc  >  1:1006629/1‑62 (MQ=255)                                  |                                     CCACAACTGCCGCAAATCAGTAGCGCCAGCCTGACGGTGGACAAACGGCATTTGCTCTATGGTAATGGGC  >  minE/1202877‑1202946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑