| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,497,293 | G→A | N83N (AAC→AAT) | nuoF ← | NADH:ubiquinone oxidoreductase, chain F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,497,293 | 0 | G | A | 100.0% | 11.5 / NA | 5 | N83N (AAC→AAT) | nuoF | NADH:ubiquinone oxidoreductase, chain F |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (5/0); total (5/0) | |||||||||||
TATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCG > minE/1497244‑1497305 | tataGGTGCCCGGCTCCATTTCATCGGCGTTACACAGTAGGTAACGGATATTCATGGATTCg > 1:4189/1‑62 (MQ=255)tataGGTGCCCGGCTCCATTTCATCGGCGTTACACAGTAGGTAACGGATATTCATGGATTCg > 1:6572/1‑62 (MQ=255)tataGGTGCCCGGCTCCATTTCATCGGCGTTACACAGTAGGTAACGGATATTCATGGATTCg > 1:728004/1‑62 (MQ=255)tataGGTGCCCGGCTCCATTTCATCGGCGTTACACAGTAGGTAACGGATATTCATGGATTCg > 1:768639/1‑62 (MQ=255)tataGGTGCCCGGCTCCATTTCATCGGCGTTACACAGTAGGTAACGGATATTCATGGATTCg > 1:774053/1‑62 (MQ=255) | TATAGGTGCCCGGCTCCATTTCATCGGCATTACACAGCAGGTAACGGATGTTCATGGATTCG > minE/1497244‑1497305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |