| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 705,870 | 0 | A | G | 42.1% | 7.9 / 20.5 | 19 | intergenic (‑367/+128) | ymcC/serX | predicted outer membrane lipoprotein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base A (2/9); new base G (5/3); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.39e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCC > minE/705845‑705937 | cgctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTACAACTGGCTGTCGCCAGTTGTCGaa < 1:2029374/69‑1 (MQ=14) gctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAccc < 1:1742088/71‑1 (MQ=34) ctcgcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAcccc < 1:983878/71‑1 (MQ=21) ctcgcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAcccc < 1:501825/71‑1 (MQ=21) cgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCgg > 1:882894/1‑71 (MQ=34) gcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCg < 1:1407092/69‑1 (MQ=35) ccTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCg > 1:1672618/1‑71 (MQ=21) ccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCg < 1:554772/71‑1 (MQ=34) gCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtc < 1:1757274/71‑1 (MQ=34) gCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtc < 1:1544474/71‑1 (MQ=34) gCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtc < 1:627013/71‑1 (MQ=34) gCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtc < 1:1541736/71‑1 (MQ=34) cAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtc < 1:779964/70‑1 (MQ=25) gCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAc > 1:511189/1‑53 (MQ=16) ccGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAATCCCGGTCGGGGCTTCTCATc > 1:159777/1‑71 (MQ=255) cGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGaa > 1:1031833/1‑49 (MQ=32) gCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATccc > 1:2384914/1‑71 (MQ=21) gCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGCGGCTtc < 1:314556/64‑1 (MQ=25) cTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcccc > 1:2300489/1‑71 (MQ=21) | CGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCC > minE/705845‑705937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |