| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,359,867 | 0 | G | A | 35.9% | 99.5 / 79.1 | 78 | noncoding (1228/2905 nt) | rrlA | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (24/26); new base A (18/10); total (42/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCG > minE/2359815‑2359934 | cccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:1544043/1‑71 (MQ=9) ccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca < 1:240262/70‑1 (MQ=31) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTGCAGCATGCCTCACAGCACACCTTCACAg > 1:190410/1‑71 (MQ=21) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCATCATGCCTCACAGCACACCTTCACAg > 1:1130353/1‑71 (MQ=21) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAg > 1:327724/1‑71 (MQ=34) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg < 1:168356/71‑1 (MQ=255) cTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAgg > 1:1211336/1‑71 (MQ=34) tttATCGTTACTTATGACAGCATTCTCACTTATGATACCTCCAGCATGCCTCACAGCACACCTTCACAgg > 1:1192818/1‑70 (MQ=11) ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt > 1:881155/1‑71 (MQ=255) aTCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcaca > 1:2320836/1‑65 (MQ=35) aTCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCtt < 1:509702/70‑1 (MQ=255) tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTAc < 1:765026/71‑1 (MQ=255) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACa < 1:1691612/70‑1 (MQ=35) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:2076606/71‑1 (MQ=34) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:184195/71‑1 (MQ=34) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTa < 1:2046372/68‑1 (MQ=255) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGc > 1:1846034/1‑62 (MQ=35) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGc > 1:1751694/1‑62 (MQ=35) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGc > 1:1580867/1‑62 (MQ=35) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGc > 1:641685/1‑62 (MQ=35) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGaa < 1:1016382/70‑1 (MQ=255) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAAc > 1:2258860/1‑71 (MQ=255) cTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACg < 1:271315/71‑1 (MQ=34) cTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:2048281/1‑56 (MQ=11) tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc < 1:534699/70‑1 (MQ=35) tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc < 1:1128502/70‑1 (MQ=35) gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc > 1:394107/1‑71 (MQ=34) gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc > 1:2325664/1‑71 (MQ=34) gTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTccc > 1:1670198/1‑71 (MQ=255) tCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca < 1:478644/50‑1 (MQ=11) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTa > 1:259972/1‑71 (MQ=34) aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACCCTCCCCt < 1:1267579/70‑1 (MQ=25) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCt > 1:168796/1‑65 (MQ=11) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCt > 1:165807/1‑65 (MQ=11) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCt > 1:612125/1‑65 (MQ=11) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa > 1:1028352/1‑71 (MQ=255) gCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACa > 1:1259725/1‑57 (MQ=35) gCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAc < 1:491382/71‑1 (MQ=34) gCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAc < 1:471056/71‑1 (MQ=34) gCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAc < 1:851242/71‑1 (MQ=34) gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt < 1:1916265/53‑1 (MQ=11) gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc > 1:434990/1‑71 (MQ=255) cATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:45435/1‑71 (MQ=255) cATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:2083635/1‑71 (MQ=255) cATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:1330736/1‑71 (MQ=255) tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTAc < 1:1897716/52‑1 (MQ=35) tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTAc < 1:353913/52‑1 (MQ=35) tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTAc < 1:458668/52‑1 (MQ=35) tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTAc < 1:1464835/52‑1 (MQ=35) tCGCACATCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTccc > 1:1800/1‑63 (MQ=11) cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCt > 1:1182083/1‑59 (MQ=35) gCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac < 1:1481976/70‑1 (MQ=35) gCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac < 1:631445/70‑1 (MQ=35) gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac < 1:1566882/70‑1 (MQ=255) aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc > 1:1127678/1‑59 (MQ=35) aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc > 1:1006831/1‑59 (MQ=35) aCTTCTCATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:3060/1‑57 (MQ=11) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg > 1:1560134/1‑69 (MQ=11) tCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca > 1:705443/1‑71 (MQ=39) tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACa > 1:1941963/1‑46 (MQ=11) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca > 1:867568/1‑70 (MQ=40) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca < 1:1014025/70‑1 (MQ=40) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca < 1:1871677/70‑1 (MQ=40) aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATa < 1:2430768/69‑1 (MQ=40) aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgt > 1:742668/1‑71 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:1413208/71‑1 (MQ=255) aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt > 1:979347/1‑71 (MQ=255) ccTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGa < 1:98624/41‑1 (MQ=14) ccTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGa < 1:416633/41‑1 (MQ=14) cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa > 1:1684020/1‑59 (MQ=35) cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCg < 1:1782875/71‑1 (MQ=255) cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCg < 1:1189538/71‑1 (MQ=255) cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCg < 1:2114114/71‑1 (MQ=255) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt > 1:1578569/1‑71 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:1479757/70‑1 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg > 1:2131936/1‑71 (MQ=255) aGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTccc > 1:2074426/1‑44 (MQ=11) cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCg < 1:1723320/71‑1 (MQ=255) gCCTCACAGCACCCCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:1127842/1‑44 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:2112582/1‑44 (MQ=38) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:946393/1‑44 (MQ=38) | CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCG > minE/2359815‑2359934 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |