Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 942,255 C→T R296K (AGG→AAG)  ddpF ← D‑Ala‑D‑Ala transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE942,2550CT93.0% 124.1 / 1.6 43R296K (AGG→AAG) ddpFD‑Ala‑D‑Ala transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (21/19);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAAC  >  minE/942189‑942321
                                                                  |                                                                  
gAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTAt                                                                >  1:2337385/1‑71 (MQ=255)
 aaTGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTAtt                                                               >  1:545833/1‑71 (MQ=255)
     ccTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTGACGTCCGTCCTCCTTTATTGCTa                                                           <  1:208832/71‑1 (MQ=255)
      cTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTaa                                                          >  1:506914/1‑71 (MQ=255)
        gATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAAt                                                         >  1:957568/1‑70 (MQ=255)
        gATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTa                                                                 <  1:272043/62‑1 (MQ=255)
            cGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTAt                                                                >  1:2393252/1‑59 (MQ=255)
            cGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTg                                                    >  1:1086682/1‑71 (MQ=255)
             gttgttAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGc                                                   >  1:101278/1‑71 (MQ=255)
                gttAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGa                                                       >  1:2238130/1‑64 (MQ=255)
                    gCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACtt                                            <  1:2373032/71‑1 (MQ=255)
                       tctcTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTca                                          >  1:1600572/1‑70 (MQ=255)
                         tctACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTa                                                                 >  1:536573/1‑45 (MQ=255)
                         tctACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTca                                          <  1:1632881/68‑1 (MQ=255)
                         tctACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACAt                                       >  1:380184/1‑71 (MQ=255)
                              aGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCt                                                            <  1:1740214/45‑1 (MQ=255)
                                  cccgccAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATcc                                     >  1:1941646/1‑64 (MQ=255)
                                     gccAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACtt                                            <  1:2168391/54‑1 (MQ=255)
                                     gccAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACtt                                            <  1:1416977/54‑1 (MQ=255)
                                     gccAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGc                            >  1:650864/1‑70 (MQ=255)
                                      ccAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGa                                               <  1:1171938/50‑1 (MQ=255)
                                      ccAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGc                            >  1:1049504/1‑69 (MQ=255)
                                       cAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAg                         <  1:332275/71‑1 (MQ=255)
                                       cAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAg                         <  1:605279/71‑1 (MQ=255)
                                       cAGCACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAg                         <  1:2186043/71‑1 (MQ=255)
                                          cACCGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCgg                      >  1:2068053/1‑71 (MQ=255)
                                            ccGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGc                    <  1:266581/71‑1 (MQ=255)
                                             cGGAGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGc                    <  1:1212654/70‑1 (MQ=255)
                                              ggAGCTCACGTCCGTGCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCCGCGGGCa                   >  1:1093260/1‑70 (MQ=255)
                                              ggAGCTCACGTCCGTCCTCCTTTATTGCTAATGAt                                                      >  1:1724370/1‑35 (MQ=255)
                                                aGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACg                <  1:552609/71‑1 (MQ=255)
                                                aGCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACg                <  1:2008761/71‑1 (MQ=255)
                                                 gCTCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGt               <  1:728528/71‑1 (MQ=255)
                                                   tCACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGc                        <  1:938392/60‑1 (MQ=255)
                                                    cACGTCCGTCCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCg            >  1:1712626/1‑71 (MQ=255)
                                                      cgtccgtcCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCg            >  1:515556/1‑69 (MQ=255)
                                                      cgtccgtcCTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCg            >  1:2381104/1‑69 (MQ=255)
                                                       gtccgtccTCCTTTATTGCTAATGATTGCCGGACTTCAAATCCgtgtgt                               >  1:186149/1‑49 (MQ=255)
                                                         ccgtccTCCTTTATTGCTAATGATTGCCGTACTTCACATcc                                     <  1:32664/41‑1 (MQ=38)
                                                         ccgtccTCCTTTATTGCTAATGATTGCCGGACTTCACATcc                                     <  1:376230/41‑1 (MQ=255)
                                                          cgtccTCCTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGaaa      <  1:633242/71‑1 (MQ=255)
                                                              ctccTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGaaaaaa   >  1:528390/1‑70 (MQ=255)
                                                              ctccTTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAAc  <  1:828361/71‑1 (MQ=255)
                                                                  |                                                                  
GAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAAC  >  minE/942189‑942321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: