| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,017,691 | A→G | E50G (GAA→GGA) | ydgA → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,017,691 | 0 | A | G | 95.1% | 138.6 / ‑1.3 | 41 | E50G (GAA→GGA) | ydgA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (13/26); total (14/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
AACCCATCTCGAAGACATGGTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGAATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTG > minE/1017632‑1017758 | aaCCCATCTCGAAGACATGGTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTg > 1:2066694/1‑70 (MQ=255) aCCCATCTCGAAGACATGGTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTgg > 1:2059297/1‑70 (MQ=255) cATCTCGAAGACATGGTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGt < 1:1268780/71‑1 (MQ=255) cATCTCGAAGACATGGTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGt < 1:2377/71‑1 (MQ=255) aTCTCGAAGACATGGTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGAATCCAACCTGGAAGt > 1:2074397/1‑70 (MQ=255) cGAAGACATGGTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGt > 1:204373/1‑70 (MQ=255) gAAGACATGGTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATcc < 1:697060/54‑1 (MQ=255) gAAGACATGGTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAg < 1:595869/68‑1 (MQ=255) aGACATGGTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCaa > 1:202822/1‑54 (MQ=255) ggTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTAt < 1:2202351/64‑1 (MQ=255) gTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACt < 1:898538/70‑1 (MQ=255) gTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACt < 1:2232911/70‑1 (MQ=255) gTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACt < 1:1730951/70‑1 (MQ=255) ttgcgcAGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCaaa < 1:1137612/64‑1 (MQ=255) aGGCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGaa > 1:1092540/1‑47 (MQ=255) gCGAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGtt > 1:1774474/1‑52 (MQ=255) gAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGtt < 1:539414/50‑1 (MQ=255) gAACGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCaaaa > 1:2378874/1‑57 (MQ=255) aaCGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGt < 1:2132201/71‑1 (MQ=255) aCGCGCAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTAt > 1:1693290/1‑50 (MQ=255) gcgcAACTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTAtt < 1:1887345/71‑1 (MQ=255) aCTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTcag > 1:1925052/1‑69 (MQ=255) aCTCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTcag > 1:577762/1‑69 (MQ=255) tCAAACTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATc < 1:1403556/42‑1 (MQ=255) aaaCTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACt < 1:1722069/46‑1 (MQ=255) aaaCTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCagcagc > 1:1422031/1‑69 (MQ=255) aaaCTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCagcagc > 1:575847/1‑69 (MQ=255) aaaCTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGcc < 1:1365124/70‑1 (MQ=255) aaCTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAg < 1:1234547/71‑1 (MQ=255) aaCTGACAGCTCCTGAATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAg < 1:952516/71‑1 (MQ=255) aCTGACAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGt < 1:487590/71‑1 (MQ=255) aCAGCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCa < 1:2012538/71‑1 (MQ=255) gCTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACt < 1:579025/71‑1 (MQ=255) cTCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTg > 1:1029628/1‑71 (MQ=255) tCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATcgc < 1:1842642/43‑1 (MQ=255) tCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTcagca < 1:1903616/56‑1 (MQ=255) tCCTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTcag < 1:888738/54‑1 (MQ=255) cTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTca < 1:2133772/51‑1 (MQ=255) cTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGtt < 1:1216765/70‑1 (MQ=255) cTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGt < 1:2102787/69‑1 (MQ=255) cTGGATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTg < 1:2185763/71‑1 (MQ=255) | AACCCATCTCGAAGACATGGTCGCGCAGGCGAACGCGCAACTCAAACTGACAGCTCCTGAATCCAACCTGGAAGTGAGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTG > minE/1017632‑1017758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |