Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,036,505	G→A	A632A (GCG→GCA)	rsxC →	fused predicted 4Fe‑4S ferredoxin‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,036,505	0	G	A	100.0%	70.5 / NA	27	A632A (GCG→GCA)	rsxC	fused predicted 4Fe‑4S ferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/8);  total (19/8)

AACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC  >  minE/1036441‑1036571                                                                 |                                                                   aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAg                                                              <  1:1163415/71‑1 (MQ=21) aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCCAAGCCGCTATTGCCCGTGCCAAAGCACGCAAg                                                              <  1:927886/71‑1 (MQ=255)    cAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGcgg                                                           >  1:1567322/1‑69 (MQ=11)       aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAg                                                              >  1:2027470/1‑65 (MQ=25)        agaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                        >  1:2194157/1‑70 (MQ=18)                        cgcAAAGCCGCCGTC‑AAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc                                      >  1:1816100/1‑71 (MQ=11)                          cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc                                      <  1:1860601/70‑1 (MQ=14)                          cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCg                                     <  1:318649/71‑1 (MQ=17)                          cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCg                                     <  1:2233901/71‑1 (MQ=17)                           aaaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGa                                    <  1:926785/71‑1 (MQ=21)                              gccgccGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGcc                                 <  1:2153653/71‑1 (MQ=255)                              gccgccGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc                                  >  1:1576809/1‑70 (MQ=255)                                cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc                                  >  1:872823/1‑68 (MQ=255)                                     tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          >  1:1774160/1‑71 (MQ=255)                                        aaGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAg                       >  1:1047612/1‑71 (MQ=255)                                            cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaac                         >  1:265093/1‑65 (MQ=255)                                            cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaac                         >  1:591623/1‑65 (MQ=255)                                             gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaac                         >  1:2138187/1‑64 (MQ=255)                                                 ttGCCCGTGCCAAAGCACGCAAGCTGGAACTGCAACAGGCTAATGCCGAGCCAGa                              <  1:2267759/55‑1 (MQ=14)                                                     ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCTGAGCCAGaa                             >  1:398680/1‑52 (MQ=11)                                                      cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATACGCGCa          >  1:1494763/1‑70 (MQ=255)                                                       gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa          >  1:548857/1‑69 (MQ=255)                                                          ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgcc     >  1:1624160/1‑71 (MQ=255)                                                             aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca                        >  1:2273867/1‑49 (MQ=255)                                                             aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca                        >  1:1271668/1‑49 (MQ=255)                                                             aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  >  1:1983089/1‑71 (MQ=255)                                                             aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgc   >  1:1968714/1‑70 (MQ=255)                                                                 |                                                                   AACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC  >  minE/1036441‑1036571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑