| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,291,205 | C→T | intergenic (+320/+133) | yeeN → / ← asnW | conserved hypothetical protein/tRNA‑Asn |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,291,205 | 0 | C | T | 100.0% | 68.5 / NA | 23 | intergenic (+320/+133) | yeeN/asnW | conserved hypothetical protein/tRNA‑Asn |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/10); total (13/10) | |||||||||||
AACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGCGTGAGACTCGATTAAGCGCACGAAACACAGAAATCAAAAAACCCGGTCACTTTTTTAC > minE/1291142‑1291263 | aaCAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGAc < 1:340424/71‑1 (MQ=255) cAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTc > 1:822002/1‑71 (MQ=255) cAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTc > 1:283411/1‑71 (MQ=255) tttttGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGAtt > 1:1360013/1‑70 (MQ=255) tttGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTAAg > 1:1997302/1‑71 (MQ=255) ttGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTAAg < 1:690686/70‑1 (MQ=255) aCCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAgtgt < 1:1324848/53‑1 (MQ=255) tGCGAATCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCACGa > 1:1013039/1‑70 (MQ=255) gAATCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAgtgt < 1:1273682/46‑1 (MQ=255) aTCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTaa > 1:897650/1‑57 (MQ=255) gTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCACGAAACACAGaa < 1:2193926/71‑1 (MQ=255) tGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCACGAAaca < 1:1590440/65‑1 (MQ=255) tGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCACGAAACACAGaaa < 1:1058649/71‑1 (MQ=255) tGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCACGAAACACAGaaa < 1:96890/71‑1 (MQ=255) aTGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCACGAAACACAGAAAt > 1:1541163/1‑70 (MQ=255) tGTCTGTTGGGGAGGGACAATTTGCTCACTGAAgtgt < 1:72075/37‑1 (MQ=255) tGTCTGTTGGGGAGGGACAATTTGCTCACTGAAgtgt < 1:1361521/37‑1 (MQ=255) tGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCACGAAACACAGAAATCa < 1:1935904/71‑1 (MQ=255) tCTGTTGGGGAGGGACAATTTGCTCACTGAAgtgt < 1:349092/35‑1 (MQ=255) tCTGTTGGGGAGGGACAATTTGCTCACTGAAgtgt < 1:373160/35‑1 (MQ=255) ttGGGGAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTaa < 1:452178/44‑1 (MQ=255) ggggAGGGACAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCACGAAACACAGAAATc > 1:1396684/1‑62 (MQ=255) ggaCAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCACGAAACACAGAAATCAAAAAACCCGGTc > 1:1967466/1‑69 (MQ=255) ggaCAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCACGAAACACAGAAATCAAAAAACCCGGTc > 1:677433/1‑69 (MQ=255) aCAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCAc > 1:1904470/1‑40 (MQ=255) aCAATTTGCTCACTGAAGTGTGAGACTCGATTAAGCGCAc > 1:258188/1‑40 (MQ=255) cTCACTGAAGTGTGAGACTCGATTAAGCGCACGAAACACAGAAATCAAAAAACCCGGTCACTTTTTTAc > 1:1858003/1‑69 (MQ=255) tCACTGAAGTGTGAGACTCGATTAAGCGCACGAAAcaca < 1:1618094/39‑1 (MQ=255) tCACTGAAGTGTGAGACTCGATTAAGCGCACGAAAcaca < 1:1615419/39‑1 (MQ=255) | AACAGAGTTTTTGACCTTGCGAATCGTGATGTCTGTTGGGGAGGGACAATTTGCTCACTGAAGCGTGAGACTCGATTAAGCGCACGAAACACAGAAATCAAAAAACCCGGTCACTTTTTTAC > minE/1291142‑1291263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |