| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,560,516 | C→T | P175S (CCA→TCA) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,560,516 | 0 | C | T | 91.4% | 101.3 / 2.1 | 35 | P175S (CCA→TCA) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base C (3/0); new base T (18/14); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
AATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATC > minE/1560458‑1560579 | aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGt < 1:102244/69‑1 (MQ=255)aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTaa < 1:870821/71‑1 (MQ=255)aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTaa > 1:752404/1‑71 (MQ=255)aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTaa > 1:1419381/1‑71 (MQ=255) aTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAAt > 1:1054683/1‑71 (MQ=255) cGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATtca > 1:1175455/1‑71 (MQ=255) cGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATtca < 1:2120348/71‑1 (MQ=255) cGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATtc < 1:970023/70‑1 (MQ=255) gCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCatcaat < 1:1441202/71‑1 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCatcaat > 1:302214/1‑70 (MQ=255) tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATCAt > 1:117329/1‑71 (MQ=255) tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCAt > 1:1543113/1‑71 (MQ=255) tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCAt > 1:206455/1‑71 (MQ=255) cTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAg < 1:1436629/47‑1 (MQ=255) cTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATtca > 1:34878/1‑60 (MQ=255) tCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATtcatc < 1:1590229/61‑1 (MQ=255) tCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATtcatc < 1:752368/61‑1 (MQ=255) cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAg > 1:2230470/1‑45 (MQ=255) cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGAc > 1:2229255/1‑47 (MQ=255) ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCa < 1:107946/42‑1 (MQ=255) cAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATCATTTCCaa > 1:1009676/1‑69 (MQ=255) aaCCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATCATTTCCAAAAGc > 1:2157842/1‑70 (MQ=255) gTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAt > 1:1647907/1‑71 (MQ=255) aaTATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTa > 1:1914827/1‑70 (MQ=255) aaTATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt > 1:1919834/1‑71 (MQ=255) aaTATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt < 1:700698/71‑1 (MQ=255) atatTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtc > 1:1346657/1‑71 (MQ=255) ttGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATctct < 1:2226855/71‑1 (MQ=255) tGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATCATTTCCa > 1:1519720/1‑52 (MQ=255) gTAGCAAATTACCCCTCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtctc < 1:1194319/62‑1 (MQ=255) gCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaaca > 1:752569/1‑51 (MQ=255) cAAATTACCCCTCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt < 1:441391/68‑1 (MQ=255) ttACCCCTCAGACGAGGTAATTCATCAATCATTTCCaaaa > 1:202474/1‑40 (MQ=255) ttACCCCTCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat > 1:834827/1‑70 (MQ=255) ttACCCCTCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc < 1:2025857/71‑1 (MQ=255) ccctcAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa < 1:770853/40‑1 (MQ=255) | AATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATC > minE/1560458‑1560579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |