| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,019,887 | C→T | Q169* (CAA→TAA) | dkgA → | 2,5‑diketo‑D‑gluconate reductase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,019,887 | 0 | C | T | 98.2% | 182.4 / ‑3.9 | 57 | Q169* (CAA→TAA) | dkgA | 2,5‑diketo‑D‑gluconate reductase A |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (29/27); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
GATTGATGAAACTGGCGTGACGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAACAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGAATCCTGGAGCCCATTA > minE/2019826‑2019949 | gattgatGAAACTGGCGTGACGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGc > 1:355539/1‑71 (MQ=255)gattgatGAAACTGGCGTGACGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGc > 1:2367492/1‑71 (MQ=255) ttgatGAAACTGGCGTGACGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCt > 1:2194192/1‑70 (MQ=255) gatgaAACTGGCGTGACGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTaca > 1:102033/1‑71 (MQ=255) aaCTGGCGTGACGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTacac < 1:1898530/67‑1 (MQ=255) aaCTGGCGTGACGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCt < 1:649435/71‑1 (MQ=255) aCTGGCGTGACGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTg > 1:2226675/1‑71 (MQ=255) gCGTGACGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGaa > 1:172307/1‑70 (MQ=255) gCGTGACGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAAc > 1:1970278/1‑71 (MQ=255) cGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGaca > 1:1142331/1‑71 (MQ=255) cTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGacaca > 1:122279/1‑70 (MQ=255) cTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGacaca > 1:1271647/1‑70 (MQ=255) cTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGacaca > 1:1370624/1‑70 (MQ=255) tgtgATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGcc < 1:913238/44‑1 (MQ=255) gtgATAAACCAGATCGAACTTCATCCTCTGATGCAATAACGcc < 1:1250543/43‑1 (MQ=38) gtgATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGAcacaca > 1:676758/1‑70 (MQ=255) tgATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTgg < 1:1092282/56‑1 (MQ=255) tgATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACaa < 1:77061/70‑1 (MQ=255) tgATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACaa > 1:736416/1‑70 (MQ=255) tgATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACaa > 1:2027288/1‑70 (MQ=255) gATAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACaaaa > 1:1141683/1‑71 (MQ=255) aTAAACCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACaaaa < 1:1230532/70‑1 (MQ=255) aTAAACCAGATCGAACTTCATCCGCTGATGCAACAACGCCAGCTACACGCCTGGAACGCGACACACAAAAt < 1:1983309/71‑1 (MQ=255) aaaCCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGac > 1:1651449/1‑60 (MQ=255) aaaCCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATc > 1:175439/1‑70 (MQ=255) aaCCAGATCGAACTTCATCCGCTGATGCAATAACGc < 1:1487591/36‑1 (MQ=255) aaCCAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATcc < 1:1179318/70‑1 (MQ=255) ccAGATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGa < 1:1407958/71‑1 (MQ=255) gATCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGAcc > 1:1704792/1‑70 (MQ=255) aTCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACg < 1:1531827/40‑1 (MQ=255) aTCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACg < 1:158187/40‑1 (MQ=255) aTCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAAt < 1:2415787/62‑1 (MQ=255) aTCGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGa < 1:971143/71‑1 (MQ=255) cGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGcc > 1:1188602/1‑40 (MQ=255) cGAACTTCATCCGCTGATGCAATAACGCCAGCTACACGc > 1:700109/1‑39 (MQ=255) gAACTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGAATc < 1:164307/71‑1 (MQ=255) aaCTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAAt < 1:302719/58‑1 (MQ=255) aaCTTCATCCCCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAg < 1:2408739/62‑1 (MQ=255) aCTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGAATcc < 1:1250385/70‑1 (MQ=255) aCTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGAATc < 1:198655/69‑1 (MQ=255) aCTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGAATCCt < 1:32890/71‑1 (MQ=255) aCTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGAATCCt < 1:33555/71‑1 (MQ=255) cTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGAc > 1:1732122/1‑62 (MQ=255) cTTCATCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGAATCCTg > 1:1508194/1‑71 (MQ=255) tCCGCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGAATCCTGGAGcc < 1:2176485/71‑1 (MQ=255) gCTGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGAATCCTGGAGCCCAt < 1:2398254/71‑1 (MQ=255) cTGATGCAATACCGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGAcc > 1:625579/1‑54 (MQ=39) tGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACaaaa > 1:1563849/1‑45 (MQ=255) tGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAAt > 1:1826477/1‑46 (MQ=255) tGATGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAAt > 1:2276198/1‑46 (MQ=255) aTGCAATAACGCCAGCTACACGCCTGGAACGCGACACACaaaa < 1:2070978/43‑1 (MQ=255) aTGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATc < 1:746485/45‑1 (MQ=255) aTGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGa > 1:2274881/1‑49 (MQ=255) aTGCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGAATCCTGGAGCCCATTa < 1:2166746/69‑1 (MQ=255) gCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCa > 1:1213486/1‑45 (MQ=255) gCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCa > 1:1119045/1‑45 (MQ=255) gCAATAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGAcc < 1:2118023/49‑1 (MQ=255) | GATTGATGAAACTGGCGTGACGCCTGTGATAAACCAGATCGAACTTCATCCGCTGATGCAACAACGCCAGCTACACGCCTGGAACGCGACACACAAAATCCAGACCGAATCCTGGAGCCCATTA > minE/2019826‑2019949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |