| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,281,038 | G→A | Q95* (CAG→TAG) | fabR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,281,038 | 0 | G | A | 97.7% | 133.6 / ‑3.8 | 43 | Q95* (CAG→TAG) | fabR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (23/19); total (24/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
CTCCATAAATGTGGAGACCGAGGTGCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTGGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACG > minE/2280973‑2281102 | cTCCATAAATGTGGAGACCGAGGTGCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCa > 1:98329/1‑71 (MQ=255) tAAATGTGGAGACCGAGGTGCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGt < 1:951996/71‑1 (MQ=255) aaaTGTGGAGACCGAGGTGCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGtt > 1:2151179/1‑71 (MQ=255) gagaCCGAGGTGCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAgcg < 1:76665/56‑1 (MQ=255) gagaCCGAGGTGCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAg < 1:1812043/62‑1 (MQ=255) aCCGAGGTGCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCAt > 1:1982879/1‑71 (MQ=255) cGAGGTGCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCAtt > 1:124953/1‑70 (MQ=255) cGAGGTGCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTa < 1:1241694/71‑1 (MQ=255) gAGGTGCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCAt > 1:1042288/1‑53 (MQ=255) gCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCg > 1:2178749/1‑70 (MQ=255) cGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTg > 1:1464362/1‑53 (MQ=255) cGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTg > 1:1816666/1‑53 (MQ=255) cGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGc > 1:626037/1‑70 (MQ=255) gATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTa < 1:71444/62‑1 (MQ=255) gATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTa > 1:1439564/1‑62 (MQ=255) gATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGctct < 1:889707/71‑1 (MQ=255) aTCACACTCCCGCCTTTAGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGctct > 1:1853386/1‑70 (MQ=255) cacaCTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTg > 1:2323714/1‑48 (MQ=255) acacTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAg < 1:1979901/44‑1 (MQ=255) cTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGt < 1:51390/42‑1 (MQ=255) cTCCCGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAAc < 1:323953/58‑1 (MQ=255) ccGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCtcatc < 1:703382/65‑1 (MQ=255) ccGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAAcc < 1:1230826/69‑1 (MQ=255) ccGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCAt > 1:90231/1‑71 (MQ=255) cGCCTTTGGCGATACGCTGACGCGCCTGGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATg > 1:468005/1‑71 (MQ=255) cGCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATg < 1:1985264/71‑1 (MQ=255) gCCTTTGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATgg < 1:1638883/71‑1 (MQ=255) tGGCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCAt > 1:1476063/1‑43 (MQ=255) ggCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTaa > 1:2170655/1‑45 (MQ=255) ggCGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTaa > 1:2086893/1‑45 (MQ=255) cGATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGa < 1:159923/68‑1 (MQ=255) gATACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCa < 1:287479/65‑1 (MQ=255) tACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGt > 1:2204731/1‑71 (MQ=255) tACGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGt < 1:1097674/71‑1 (MQ=255) aCGCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGt > 1:2147401/1‑70 (MQ=255) gCTGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCg < 1:932504/71‑1 (MQ=255) tGACGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCa > 1:1536221/1‑58 (MQ=255) aCGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTc < 1:568547/69‑1 (MQ=255) aCGCGCCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTa > 1:102560/1‑71 (MQ=255) gcgcCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTc < 1:649243/67‑1 (MQ=255) gcgcCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACg > 1:1131583/1‑71 (MQ=255) gcCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCtcat > 1:1615222/1‑40 (MQ=255) gcCTAGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCt > 1:2100232/1‑66 (MQ=255) | CTCCATAAATGTGGAGACCGAGGTGCGGATCACACTCCCGCCTTTGGCGATACGCTGACGCGCCTGGCGCATGAGTTGGCGTAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACG > minE/2280973‑2281102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |