| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 86,263 | C→T | S253S (AGC→AGT) | murC → | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 86,263 | 0 | C | T | 98.2% | 168.8 / ‑3.8 | 54 | S253S (AGC→AGT) | murC | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (30/23); total (31/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
TTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGC > minE/86196‑86330 | ttGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGaa < 1:2392174/71‑1 (MQ=255) gatgatCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCg > 1:2159064/1‑67 (MQ=255) tCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGcc > 1:1053882/1‑70 (MQ=255) tCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTCACGGCTTCAGTGAAGATGcc > 1:1840687/1‑70 (MQ=255) tGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGAcgtg > 1:1028461/1‑71 (MQ=255) gATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGAcgtgc > 1:1374400/1‑71 (MQ=255) cgcgAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGAcgtgcgtg < 1:2003655/70‑1 (MQ=255) tcgAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGa > 1:1357397/2‑61 (MQ=255) cgAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGAcgtgcgtg > 1:1341795/1‑68 (MQ=255) cgAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGAcgtgcgtg > 1:1471532/1‑68 (MQ=255) cgAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTag < 1:1380652/71‑1 (MQ=255) cgAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTag < 1:1416803/71‑1 (MQ=255) cgAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTag < 1:2295719/71‑1 (MQ=255) gAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTaga < 1:2119582/71‑1 (MQ=255) ttGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATg > 1:2009247/1‑52 (MQ=255) tGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGAt > 1:1852196/1‑71 (MQ=255) tACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTAt > 1:1612817/1‑71 (MQ=255) aCCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTAt > 1:1862770/1‑70 (MQ=255) gcgAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGaa > 1:999039/1‑40 (MQ=255) gcgAGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGc < 1:1854836/45‑1 (MQ=255) aGTGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGAcgt > 1:1671803/1‑48 (MQ=255) gTGGGGCGTCAGACCACGACTTACGGCTTCAGTGaa < 1:2148884/36‑1 (MQ=255) tGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGAtt < 1:2085287/71‑1 (MQ=255) tGGGGCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGAtt < 1:1515534/71‑1 (MQ=255) ggggCGTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGAtt < 1:2082439/70‑1 (MQ=255) gTCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCg > 1:522264/1‑71 (MQ=255) tCAGACCACGACTTACGGCTTCAGTGAAGATGCCGAc < 1:417704/37‑1 (MQ=255) tCAGACCACGACTTACGGCTTCAGTGAAGATGCCGAc < 1:1244613/37‑1 (MQ=255) tCAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCg > 1:2132366/1‑70 (MQ=255) cAGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTg > 1:788571/1‑64 (MQ=255) aGACCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGa > 1:1225079/1‑60 (MQ=255) aCCACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAggg > 1:1562511/1‑71 (MQ=255) cACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTagaaga > 1:424817/1‑45 (MQ=255) cACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTagaaga > 1:1113394/1‑45 (MQ=255) cACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTagaaga > 1:2210773/1‑45 (MQ=255) cACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTagaaga > 1:93066/1‑45 (MQ=255) cACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCa < 1:2416600/66‑1 (MQ=255) cACGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGc < 1:1746847/71‑1 (MQ=255) aCGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCa > 1:419675/1‑71 (MQ=255) cGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGAt > 1:67420/1‑55 (MQ=255) cGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGccc > 1:1744946/1‑61 (MQ=255) cGACTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCAc > 1:2047910/1‑71 (MQ=255) aCTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACtt < 1:1878659/71‑1 (MQ=255) cTTACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACtt > 1:1133328/1‑70 (MQ=255) ttACGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAg < 1:272253/61‑1 (MQ=255) aCGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGAt < 1:710084/49‑1 (MQ=255) aCGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGAt < 1:1492442/49‑1 (MQ=255) aCGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGAt < 1:2184720/49‑1 (MQ=255) aCGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACtt < 1:1568033/67‑1 (MQ=255) aCGGCTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACg > 1:1505765/1‑71 (MQ=255) cTTCAGTGAAGATGCCGACGTGCGTGTAGAAGATTAt < 1:2241406/37‑1 (MQ=255) ttCAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctgct > 1:69365/1‑71 (MQ=255) cAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctgc < 1:1931697/68‑1 (MQ=255) cAGTGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCtgctgc > 1:248492/1‑71 (MQ=255) | TTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGC > minE/86196‑86330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |