| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,520,434 | G→A | W44* (TGG→TAG) | ligB → | DNA ligase, NAD(+)‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,520,434 | 0 | G | A | 98.3% | 193.8 / ‑3.9 | 60 | W44* (TGG→TAG) | ligB | DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (34/25); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TCCGGCCTGGTCGCCAGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTGGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGATCAGTTAAGCGC > minE/2520369‑2520500 | tCCGGCCTGGTCGCCAGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTagg < 1:1264625/68‑1 (MQ=255)tCCGGCCTGGTCGCCAGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGAc < 1:322581/70‑1 (MQ=255) ccGGCCTGGTCGCCAGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGa > 1:622422/1‑71 (MQ=255) gCCTGGTCGCCAGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGa > 1:1847919/1‑71 (MQ=255) cTGGTCGCCAGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGac > 1:795795/1‑70 (MQ=255) gTCGCCAGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGactact > 1:885458/1‑71 (MQ=255) gTCGCCAGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGactact > 1:1235886/1‑71 (MQ=255) ccagccagAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTgg > 1:410139/1‑69 (MQ=255) cagccagAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTggaag < 1:101218/71‑1 (MQ=255) agccagAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTggaagg < 1:469906/71‑1 (MQ=255) agccagAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTggaag > 1:252948/1‑70 (MQ=255) gccagAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTggaagga < 1:133031/71‑1 (MQ=255) ccagAGCAGAGGAAGAAATTTCCCGCCTGCAACAGCAAATTAAACAGTAGGACGAt < 1:754097/56‑1 (MQ=255) agagCACAGGAAGAAATTTCCCGCCTGCAACCGCAAATAAAACAGTAGGACGATGACTACTggaaggaag > 1:1879795/1‑70 (MQ=255) gagCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTggaaggaagg > 1:1102076/1‑70 (MQ=255) agCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTggaaggaagga > 1:1234877/1‑70 (MQ=255) agCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTggaaggaagga > 1:2313448/1‑70 (MQ=255) cacaGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGaaa > 1:794305/1‑70 (MQ=255) cacaGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGaaa > 1:1547632/1‑70 (MQ=255) caGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGa > 1:996969/1‑50 (MQ=255) caGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAg > 1:1656567/1‑71 (MQ=255) aGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGaaa < 1:306404/67‑1 (MQ=255) ggAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGaaa > 1:1854763/1‑66 (MQ=255) ggAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTg < 1:376972/71‑1 (MQ=255) aagaaATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAg < 1:1012380/71‑1 (MQ=255) aagaaATTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAg < 1:1490611/71‑1 (MQ=255) agaaATTTCCCGCCTGCAACAGCAAATAAAACAGTGGGACGATGACTACTGGAAGTAAGGAAAAAGTGAgg < 1:1539475/71‑1 (MQ=255) aaTTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTgg < 1:1607/71‑1 (MQ=255) aaTTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTg > 1:1387778/1‑70 (MQ=255) aTTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGactac < 1:2208185/45‑1 (MQ=255) aTTTCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTgg > 1:873439/1‑70 (MQ=255) tttCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTgg < 1:1530434/69‑1 (MQ=255) tttCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTgg < 1:484629/69‑1 (MQ=255) ttCCCGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTgg > 1:1530094/1‑68 (MQ=255) cccGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGa < 1:1608637/70‑1 (MQ=255) cccGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGa < 1:584296/70‑1 (MQ=255) cccGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGAc > 1:1193822/1‑71 (MQ=255) ccGCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACg < 1:1258603/71‑1 (MQ=255) gCCTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGgt > 1:2011744/1‑71 (MQ=255) ccTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGgtg > 1:1133452/1‑71 (MQ=255) ccTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGgtg > 1:1404394/1‑71 (MQ=255) cTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGaaaaa > 1:1368736/1‑52 (MQ=255) cTGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGgtgt > 1:1665582/1‑71 (MQ=255) cTGCAACAGCAAATAAAACAGTAGGACGATGACAACTGGAAGGAAGGaaaa > 1:26616/1‑51 (MQ=255) tGCAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGgtg > 1:402087/1‑69 (MQ=255) cAACAGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGtt < 1:662954/69‑1 (MQ=255) aGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGa < 1:1362362/58‑1 (MQ=255) aGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGa < 1:1255418/58‑1 (MQ=255) aGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTAc > 1:2337066/1‑68 (MQ=255) aGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGAt > 1:1274576/1‑71 (MQ=255) aGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGAt > 1:2005861/1‑71 (MQ=255) aGCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGAt > 1:143953/1‑71 (MQ=255) aGCAAAGAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGAt > 1:24/1‑71 (MQ=255) gCAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGAt > 1:1555646/1‑70 (MQ=255) cAAATAAAACAGTAGGACGATGACTACTGGAAGGAAGGaaa < 1:1127320/41‑1 (MQ=255) aTAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTgg < 1:428758/49‑1 (MQ=255) aTAAAACAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGATCAGtt < 1:1661627/71‑1 (MQ=255) aaaaCAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGa < 1:1864265/42‑1 (MQ=255) aCAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGATCAGTTAAgcg > 1:1719036/1‑71 (MQ=255) cAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGATCAGTTAAgcgc < 1:2277404/71‑1 (MQ=255) cAGTAGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGATCAGTTAAgcgc < 1:1864858/71‑1 (MQ=255) | TCCGGCCTGGTCGCCAGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTGGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTTTACGATCAGTTAAGCGC > minE/2520369‑2520500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |