| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,881,808 | C→T | V87I (GTT→ATT) | ytfG ← | NAD(P)H:quinone oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,881,808 | 0 | C | T | 94.4% | 107.2 / ‑0.8 | 36 | V87I (GTT→ATT) | ytfG | NAD(P)H:quinone oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (19/15); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
GGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTC > minE/2881740‑2881868 | ggTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAatat < 1:42912/71‑1 (MQ=255) aTCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTAc > 1:1887301/1‑71 (MQ=255) tGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGAtgctg > 1:89165/1‑71 (MQ=255) tGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGAtgctg < 1:1955529/71‑1 (MQ=255) tAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCg < 1:2280599/71‑1 (MQ=255) cTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACg < 1:999307/71‑1 (MQ=255) gTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGtt < 1:1608356/70‑1 (MQ=255) gTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGtt < 1:528177/70‑1 (MQ=255) gTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGtt < 1:289438/70‑1 (MQ=255) taAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGAcc > 1:1633069/1‑71 (MQ=255) cGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACt > 1:1208572/1‑71 (MQ=255) cGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACt > 1:1463908/1‑71 (MQ=255) aaTTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGa > 1:1862086/1‑60 (MQ=255) aaTTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCt > 1:976830/1‑71 (MQ=255) aTTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTg > 1:184702/1‑71 (MQ=255) aTTTCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTg > 1:543395/1‑71 (MQ=255) tttCACGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTgg < 1:2396401/71‑1 (MQ=255) aCGCCAGCCGCCTTTGCGGCATTAATAATATTACTATGCTGCGGGGCACGTTGACCCACTTCGCTGGaa > 1:1967802/1‑69 (MQ=255) cGCCAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGaa > 1:672983/1‑68 (MQ=255) cAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGAt > 1:699063/1‑70 (MQ=255) cAGCCGCCTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATc < 1:257941/71‑1 (MQ=255) gccgccTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATcag > 1:153595/1‑71 (MQ=255) gccgccTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATcag < 1:376502/71‑1 (MQ=255) gccTTTGCGGCATTAATAATATTACGATGCTGCGGGGCAc < 1:164550/40‑1 (MQ=255) gccTTTGCGGCATTAATAATATTACGATGCTGCGGGGCAc < 1:2304628/40‑1 (MQ=255) gccTTTGCGGCATTAATAATATTACGATGCTGCGGGGCAc < 1:1468879/40‑1 (MQ=255) gccTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATcagcag > 1:193847/1‑71 (MQ=255) cTTTGCGGCATTAATAATATTACGATGCTGCGGGGCACGt > 1:896714/1‑40 (MQ=255) ttGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTg < 1:1755801/40‑1 (MQ=255) ttGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCagtagt > 1:1448579/1‑71 (MQ=255) ttGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCagtagt > 1:1921805/1‑71 (MQ=255) tGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACtt > 1:1551135/1‑47 (MQ=255) tGCGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGtt > 1:635067/1‑71 (MQ=255) gCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGAc > 1:899498/1‑40 (MQ=255) cGGCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGAAGtttt < 1:318294/71‑1 (MQ=255) gCATTAATAATATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTc < 1:1335607/71‑1 (MQ=255) taataaTATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAgaga > 1:733793/1‑51 (MQ=255) | GGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTC > minE/2881740‑2881868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |