| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,891,553 | A→G | K70E (AAA→GAA) | ytfQ → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,891,553 | 0 | A | G | 98.7% | 295.3 / ‑4.2 | 79 | K70E (AAA→GAA) | ytfQ | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (22/56); total (22/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
AGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGG > minE/2891484‑2891620 | aGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTa > 1:2419427/1‑70 (MQ=255) tGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCATCAAAAGCAGGAAAACCAGATTGAAg < 1:1137783/71‑1 (MQ=255) tGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAg < 1:2298690/71‑1 (MQ=255) aaGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCg < 1:586258/71‑1 (MQ=255) aaGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCg < 1:1582270/71‑1 (MQ=255) aGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCgg < 1:31038/71‑1 (MQ=255) aGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCgg < 1:162134/71‑1 (MQ=255) aaaaGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGt < 1:1906930/71‑1 (MQ=255) aaaGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGtt > 1:1239176/1‑71 (MQ=255) aaGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTc > 1:1464944/1‑71 (MQ=255) cgcgGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCtt < 1:1718802/71‑1 (MQ=255) cgGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGc < 1:1267474/57‑1 (MQ=255) cgGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGc < 1:1380924/57‑1 (MQ=255) gAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGtt < 1:2211594/71‑1 (MQ=255) tCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGca > 1:2115219/1‑71 (MQ=255) tCACGATGAAAATTGGCGATGGTCAGCTAAAGCAGGAAAACCAGATTGAAg > 1:441685/1‑51 (MQ=37) aCGTTGAAAATTGCCGTTGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGcac > 1:642834/1‑70 (MQ=255) aCGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCtt > 1:1774442/1‑62 (MQ=255) aCGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCtt > 1:513156/1‑62 (MQ=255) aCGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGcac < 1:924887/70‑1 (MQ=255) aCGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGcac < 1:765364/70‑1 (MQ=255) cGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGcac > 1:565890/1‑69 (MQ=255) cGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGcac > 1:1797447/1‑69 (MQ=255) cGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACaa < 1:1860802/71‑1 (MQ=255) gAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAgggg > 1:1015414/1‑71 (MQ=255) gAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAgggg > 1:931112/1‑71 (MQ=255) aaTTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTa > 1:333712/1‑46 (MQ=255) aaTTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTa > 1:497766/1‑46 (MQ=255) aTTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAg < 1:2161100/40‑1 (MQ=255) aTTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAg < 1:2095407/40‑1 (MQ=255) aTTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGa < 1:1612174/71‑1 (MQ=255) aTTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGa < 1:2215208/71‑1 (MQ=255) aTTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGa < 1:1104832/71‑1 (MQ=255) aTTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGa < 1:423406/71‑1 (MQ=255) ttGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGAt < 1:262598/71‑1 (MQ=255) gCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGcac < 1:709171/58‑1 (MQ=255) gCCGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGcac < 1:1690764/58‑1 (MQ=255) ccGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAggg < 1:1546153/62‑1 (MQ=255) ccGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAggg < 1:440802/62‑1 (MQ=255) cGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTAc < 1:1986793/41‑1 (MQ=255) cGATGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAggg < 1:905849/61‑1 (MQ=255) aTGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGt < 1:1963914/37‑1 (MQ=255) aTGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTAc > 1:604560/1‑39 (MQ=255) aTGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATc > 1:1878437/1‑71 (MQ=255) tGGTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCt > 1:290190/1‑44 (MQ=255) gTCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCttt < 1:1800322/71‑1 (MQ=255) tCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCtt < 1:2429735/42‑1 (MQ=255) tCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCtt < 1:1123749/42‑1 (MQ=255) tCAGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCtt < 1:740767/42‑1 (MQ=255) aGCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATc > 1:201218/1‑71 (MQ=255) gCAAAAGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAggg < 1:1029127/52‑1 (MQ=255) cAAAAGCAGGAAAACTAGATTGAAGCGGTACGTTCCTTCg < 1:878433/40‑1 (MQ=37) aaaaGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGc < 1:392003/70‑1 (MQ=255) aaaaGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGc < 1:1810811/70‑1 (MQ=255) aaaaGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCt < 1:925480/71‑1 (MQ=255) aaGCTGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTcc < 1:2301602/71‑1 (MQ=255) aaGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAGGGGGTGGATGCGATCTTTATCGCTcc < 1:2010806/71‑1 (MQ=255) aaGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTcc < 1:1405033/71‑1 (MQ=255) aaGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTcc < 1:768077/71‑1 (MQ=255) aaGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTcc < 1:1782451/71‑1 (MQ=255) aaGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTcc < 1:1203835/71‑1 (MQ=255) aGCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCt < 1:1564034/68‑1 (MQ=255) gCAGGAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCg < 1:1577075/55‑1 (MQ=255) ggAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCggtgg < 1:899541/71‑1 (MQ=255) gAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGcac < 1:149595/37‑1 (MQ=255) gAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACaa < 1:909881/39‑1 (MQ=255) gAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACaa < 1:107889/39‑1 (MQ=255) gAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCt < 1:524267/55‑1 (MQ=255) gAAAACCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCggtggt > 1:6121/1‑71 (MQ=255) aaaCCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCg < 1:840776/49‑1 (MQ=255) aaaCCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTAt < 1:641143/57‑1 (MQ=255) aaaCCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTAt < 1:1801813/57‑1 (MQ=255) aaaCCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTcc > 1:94625/1‑63 (MQ=255) aaaCCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTcc > 1:1596824/1‑63 (MQ=255) aaaCCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTcc > 1:1645457/1‑63 (MQ=255) aaCCAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTcgc < 1:887585/71‑1 (MQ=255) cAGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGa > 1:2362012/1‑70 (MQ=255) aGATTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGa < 1:224629/69‑1 (MQ=255) aTTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTc < 1:862751/63‑1 (MQ=255) aTTGAAGCGGTACGTTCCTTCGTTGCACAAGGGGTAGATGCGATCTTTATCGCTCCGGTGGTCGCGACAgg < 1:500987/71‑1 (MQ=255) | AGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGG > minE/2891484‑2891620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |