Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	204,556	A→G	intergenic (+156/‑28)	alaV → / → rrlH	tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	204,556	0	A	G	100.0%	237.0 / NA	73	intergenic (+156/‑28)	alaV/rrlH	tRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/32);  total (41/32)

AAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT  >  minE/204492‑204623                                                                 |                                                                    aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                                <  1:1360654/70‑1 (MQ=255) aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                                <  1:2388017/70‑1 (MQ=255)  aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                                >  1:1712280/1‑69 (MQ=255)  aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                                >  1:130284/1‑69 (MQ=255)  aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                                >  1:1416108/1‑69 (MQ=255)      gAAACGCTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa                                                           <  1:380168/70‑1 (MQ=11)      gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa                                                          >  1:1826430/1‑71 (MQ=255)      gAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa                                                          >  1:1931874/1‑71 (MQ=255)         acacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACa                                                        >  1:1540292/1‑70 (MQ=255)            cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt                                                    <  1:843365/71‑1 (MQ=255)                 caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt                                               >  1:1163040/1‑71 (MQ=255)                   atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                                                                <  1:1140855/50‑1 (MQ=255)                   atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg                                             <  1:378207/69‑1 (MQ=255)                   atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg                                             >  1:2177495/3‑71 (MQ=255)                   atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg                                             >  1:2312142/3‑71 (MQ=255)                   atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg                                             <  1:375214/69‑1 (MQ=255)                   atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg                                             >  1:61521/3‑71 (MQ=255)                    tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                            >  1:157284/2‑71 (MQ=19)                    tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                            >  1:781739/2‑71 (MQ=19)                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                           <  1:1404464/70‑1 (MQ=25)                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                           <  1:951357/70‑1 (MQ=25)                      aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt                                            >  1:1164845/1‑69 (MQ=25)                       aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                                         >  1:204719/1‑71 (MQ=21)                          ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                                      <  1:1999595/71‑1 (MQ=21)                             ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                           <  1:478724/63‑1 (MQ=25)                             ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAg                                   >  1:1497872/1‑71 (MQ=21)                              tCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                                  >  1:1635921/1‑71 (MQ=21)                                gTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGa                                >  1:1063513/1‑71 (MQ=21)                                  gAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaa                                                           <  1:998891/42‑1 (MQ=255)                                   aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt                                      >  1:34426/1‑62 (MQ=25)                                   aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                              >  1:913838/1‑70 (MQ=25)                                   aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt                              >  1:2398069/1‑70 (MQ=25)                                      ctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                           <  1:666591/54‑1 (MQ=255)                                       tctcAAATTTTCGCAACACGATGATGGATCGCAAGa                                                            >  1:1669369/1‑36 (MQ=255)                                       tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGc                          >  1:2352095/1‑70 (MQ=25)                                       tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                         >  1:1697224/1‑71 (MQ=21)                                        ctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTaa                                    <  1:106427/59‑1 (MQ=255)                                        ctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTaa                                    <  1:857201/59‑1 (MQ=255)                                        ctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTaa                                    <  1:613287/59‑1 (MQ=255)                                        ctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTaa                                    <  1:1408362/59‑1 (MQ=255)                                         tcAAATTTTCGCAACACGATGATGGATCGCAAGaa                                                           <  1:1366019/35‑1 (MQ=255)                                         tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                                  <  1:626555/60‑1 (MQ=255)                                          cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGtt                                              <  1:1782249/47‑1 (MQ=255)                                          cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                                  >  1:2083571/1‑59 (MQ=255)                                          cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc                                  >  1:383148/1‑59 (MQ=255)                                          cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa                       >  1:1451402/1‑70 (MQ=25)                                           aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAg                                         >  1:2398921/1‑51 (MQ=255)                                            aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg                                                <  1:1342415/43‑1 (MQ=255)                                            aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac                    <  1:906918/71‑1 (MQ=21)                                             aTTTTCGCAACACGATGATGGATCGCAAGAAACATCt                                                     >  1:483362/1‑37 (MQ=255)                                             aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                     >  1:1494585/1‑69 (MQ=25)                                             aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                     >  1:206108/1‑69 (MQ=25)                                             aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca                     >  1:1664426/1‑69 (MQ=25)                                             aTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACg                   >  1:1470795/1‑71 (MQ=21)                                              ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAg                           <  1:1612079/62‑1 (MQ=25)                                              ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGc                          >  1:1285983/1‑63 (MQ=25)                                              ttttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGc                          >  1:1791315/1‑63 (MQ=25)                                                ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt                 <  1:881602/70‑1 (MQ=25)                                                ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg                >  1:1710323/1‑71 (MQ=21)                                                ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg                >  1:212751/1‑71 (MQ=21)                                                  cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGtt                                              <  1:1723429/39‑1 (MQ=255)                                                  cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa              >  1:337051/1‑71 (MQ=21)                                                     aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc           >  1:1688846/1‑71 (MQ=21)                                                      acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc          <  1:464733/71‑1 (MQ=21)                                                      acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc          <  1:1110785/71‑1 (MQ=21)                                                       cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc         <  1:1306435/71‑1 (MQ=21)                                                       cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc         >  1:1809848/1‑71 (MQ=21)                                                       cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc         <  1:298793/71‑1 (MQ=21)                                                       cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGccc         >  1:1770558/1‑71 (MQ=21)                                                        acGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt        <  1:2015741/71‑1 (MQ=21)                                                         cGATGATGGATCGCAAGAAACATCTTCGGGTtgtg                                           <  1:1122696/35‑1 (MQ=255)                                                          gatgatGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg      <  1:424199/71‑1 (MQ=21)                                                           atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt                 >  1:319091/1‑59 (MQ=255)                                                            tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                         <  1:699541/50‑1 (MQ=255)                                                            tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                         <  1:374793/50‑1 (MQ=255)                                                            tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg                         <  1:2128933/50‑1 (MQ=255)                                                              atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGCCTAAGCGTACACGGTGGATGCCCTGGCAGt  >  1:227831/1‑71 (MQ=255)                                                              atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGc                          <  1:509611/47‑1 (MQ=255)                                                              atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGc                          <  1:482129/47‑1 (MQ=255)                                                                 |                                                                    AAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT  >  minE/204492‑204623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑