| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 498,954 | C→T | Q430Q (CAG→CAA) | modF ← | fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 498,954 | 0 | C | T | 93.4% | 186.6 / 3.9 | 61 | Q430Q (CAG→CAA) | modF | fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base T (24/33); total (25/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
GTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGC > minE/498886‑499016 | gTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTg < 1:591563/71‑1 (MQ=255) tttCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCATCGGATCAAGCCCTTGt < 1:2280382/71‑1 (MQ=255) tttCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTg < 1:2376055/70‑1 (MQ=255) tttCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGt < 1:459024/71‑1 (MQ=255) tCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAg > 1:1124280/1‑71 (MQ=255) cACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAg < 1:917083/70‑1 (MQ=255) cACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGt > 1:59564/1‑71 (MQ=255) aCCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGt < 1:2396725/70‑1 (MQ=255) ttCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGtt > 1:2394701/1‑71 (MQ=255) gCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCa < 1:1745639/70‑1 (MQ=255) gCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCAt < 1:222156/71‑1 (MQ=255) aaTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATc > 1:305181/1‑69 (MQ=255) aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATc > 1:61081/1‑68 (MQ=255) aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCga < 1:127925/70‑1 (MQ=255) aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCga < 1:125066/70‑1 (MQ=255) aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCga < 1:1985769/70‑1 (MQ=255) aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCg < 1:1949386/69‑1 (MQ=255) tCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCgaga < 1:2105326/71‑1 (MQ=255) aGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGaa > 1:221492/1‑70 (MQ=255) aGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAAt < 1:1679124/71‑1 (MQ=255) aTCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAg > 1:1482065/1‑69 (MQ=255) tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAAt < 1:150089/65‑1 (MQ=255) acaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGt < 1:219365/71‑1 (MQ=255) aaaCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCaa > 1:1636075/1‑65 (MQ=255) aaaCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCgg < 1:919783/71‑1 (MQ=255) aCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCAt < 1:170521/50‑1 (MQ=255) aCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCAt < 1:415472/50‑1 (MQ=255) aCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCAt < 1:2201913/50‑1 (MQ=255) ggcggATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCg > 1:1114652/1‑50 (MQ=255) gcggATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGtt < 1:2159382/71‑1 (MQ=255) aTAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCAc > 1:2361019/1‑71 (MQ=255) aTAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCAc > 1:2291874/1‑71 (MQ=255) aTAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCAc > 1:1036353/1‑71 (MQ=255) tAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCAc > 1:2064602/1‑70 (MQ=255) aGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATc < 1:1842507/41‑1 (MQ=255) gCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCgaga < 1:228287/44‑1 (MQ=255) gCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCg < 1:250471/57‑1 (MQ=255) ggCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGt < 1:418092/52‑1 (MQ=255) ggCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGt < 1:237503/52‑1 (MQ=255) gCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTgcg < 1:2038997/70‑1 (MQ=255) gCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTgc < 1:1980505/69‑1 (MQ=255) gCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATaa < 1:1999394/44‑1 (MQ=255) gATTCAGCGGATCAACCCCCTGTAGTGGTTCATCGAGAATAAGc > 1:6447/1‑44 (MQ=255) ttCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGt > 1:184186/1‑47 (MQ=255) ttCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGt > 1:2316306/1‑47 (MQ=255) ttCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAg < 1:834618/62‑1 (MQ=255) ttCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAg < 1:1151220/62‑1 (MQ=255) tCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGttt > 1:410612/1‑55 (MQ=255) tCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGttt > 1:554907/1‑55 (MQ=255) aGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACaa < 1:2193431/70‑1 (MQ=255) aGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAAt < 1:2208218/71‑1 (MQ=255) cGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAAt > 1:993457/1‑69 (MQ=255) ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGAt > 1:1901162/1‑46 (MQ=255) ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCa > 1:1429030/1‑52 (MQ=255) ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCAc > 1:776653/1‑53 (MQ=255) ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACa > 1:485663/1‑66 (MQ=255) ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAAt > 1:750571/1‑68 (MQ=255) ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAg < 1:2065259/71‑1 (MQ=255) gATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAg > 1:1052617/1‑70 (MQ=255) tCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAgcgc < 1:1458559/71‑1 (MQ=255) gCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTgcgc < 1:1053787/54‑1 (MQ=255) | GTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGC > minE/498886‑499016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |