| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 694,703 | G→A | G89S (GGC→AGC) | appC → | cytochrome bd‑II oxidase, subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 694,703 | 0 | G | A | 90.3% | 86.7 / 2.2 | 31 | G89S (GGC→AGC) | appC | cytochrome bd‑II oxidase, subunit I |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (15/13); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
GGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAATGGCCTTCTTCCTCGAATCCACCTTTGTCG > minE/694639‑694772 | ggCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGata > 1:1614973/1‑71 (MQ=255) cTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATAtt < 1:250008/71‑1 (MQ=255) cTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATAtt < 1:649321/71‑1 (MQ=255) tACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATAtt > 1:2351090/1‑70 (MQ=255) aCCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATAtttt > 1:1007561/1‑71 (MQ=255) ccGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATAttttt < 1:767213/71‑1 (MQ=255) ccGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATAtttt < 1:1207586/70‑1 (MQ=255) gCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGgc < 1:822141/71‑1 (MQ=255) gCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATAttttt > 1:993360/1‑68 (MQ=255) gCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGgc > 1:1805609/1‑71 (MQ=255) gACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCGCAc > 1:1914589/1‑71 (MQ=255) ccATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCGCACCg < 1:197857/71‑1 (MQ=255) ccATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCGCACCg < 1:2257341/71‑1 (MQ=255) ggAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAAATATGTGAGCGATAttttt > 1:2077011/1‑58 (MQ=37) cAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGCGATgg < 1:784478/70‑1 (MQ=255) tttGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGCGATGGAAg > 1:1521001/1‑70 (MQ=255) ttGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGc < 1:2147696/61‑1 (MQ=255) ttGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGc < 1:270885/61‑1 (MQ=255) tGGTACTAACTGGTCATTCTATTCCAACTATGTGAGCTATATTTTTGGCGCACCGCTGGCGATGGAAGCAt > 1:926898/1‑71 (MQ=255) aCTAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCTCACCGCTGGCGATGGAAGCATTaa < 1:536026/70‑1 (MQ=255) cTAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAAt < 1:419908/70‑1 (MQ=255) cTAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAATg > 1:549215/1‑71 (MQ=255) tAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGCGATgg < 1:2112136/59‑1 (MQ=255) tAACTGGTCATTCTATTCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAATgg > 1:1520958/1‑71 (MQ=255) aaCTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAATGGc > 1:480735/1‑71 (MQ=255) ggTCATTCTATTCCAACTATGTGAGCGATATTTTTg > 1:575335/1‑36 (MQ=255) cTATTCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGCGATgg > 1:1808386/1‑47 (MQ=255) tATTCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGCGATGGaa < 1:2359441/48‑1 (MQ=255) ttCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGCGa > 1:1862464/1‑41 (MQ=255) ttCCAACTATGTGAGCGATATTTTTGGCGCACCGCTGGCGATGGAAGc > 1:1121872/1‑48 (MQ=255) cAACTATGTGAGCGATATTTTTGGCGCACCGCTGGCGAt < 1:1127711/39‑1 (MQ=255) ggCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAATGGCCTTCTTCCTCGAATCCACCTTTGTCg < 1:740778/70‑1 (MQ=255) | GGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAATGGCCTTCTTCCTCGAATCCACCTTTGTCG > minE/694639‑694772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |