Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | minE | 817,564 | C→T | L113L (CTC→CTT) | prfA → | peptide chain release factor RF‑1 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 817,564 | 0 | C | T | 95.7% | 140.9 / ‑1.2 | 47 | L113L (CTC→CTT) | prfA | peptide chain release factor RF‑1 |
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (23/22); total (24/23) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 |
CAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGC > minE/817496‑817633 | cAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGa < 1:968161/71‑1 (MQ=255) cAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGa < 1:1538685/71‑1 (MQ=255) cTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGt > 1:21663/1‑71 (MQ=255) gAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc < 1:832632/69‑1 (MQ=255) aTTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGaa > 1:556705/1‑71 (MQ=255) aTTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGaa > 1:229676/1‑71 (MQ=255) ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc < 1:2322269/71‑1 (MQ=255) ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc > 1:711993/1‑71 (MQ=255) ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc > 1:1714820/1‑71 (MQ=255) ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGaa < 1:831734/57‑1 (MQ=255) aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAcc > 1:956262/1‑70 (MQ=255) aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg > 1:2419422/1‑71 (MQ=255) aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg > 1:2208722/1‑71 (MQ=255) aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg > 1:1205817/1‑71 (MQ=255) gTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGcc > 1:1825407/1‑60 (MQ=255) gTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcgg < 1:791117/68‑1 (MQ=255) gTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg < 1:1323525/67‑1 (MQ=255) ttCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCggcggc < 1:696453/71‑1 (MQ=255) gTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc < 1:579702/49‑1 (MQ=255) gTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc < 1:1254546/49‑1 (MQ=255) ttACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGcgacga < 1:949029/71‑1 (MQ=255) aCTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGaa > 1:626153/1‑70 (MQ=255) cTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAg < 1:243170/70‑1 (MQ=255) gCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGt < 1:1556713/42‑1 (MQ=255) ccAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc > 1:1794142/1‑53 (MQ=255) aaaGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAgcgg < 1:1787157/67‑1 (MQ=255) aGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg > 1:1227636/1‑50 (MQ=255) gATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc > 1:2049883/1‑36 (MQ=255) gATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc > 1:1395202/1‑36 (MQ=255) cTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTcgcg < 1:2406752/71‑1 (MQ=255) cTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTcgc < 1:1971296/70‑1 (MQ=255) atgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcggc > 1:2105485/1‑44 (MQ=255) atgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGc > 1:1777498/1‑71 (MQ=255) tgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCgg < 1:2341330/68‑1 (MQ=255) tgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGc > 1:1831853/1‑70 (MQ=255) tgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCg < 1:658994/71‑1 (MQ=255) aCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCg < 1:1697700/69‑1 (MQ=255) aCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGa < 1:1768541/70‑1 (MQ=255) cGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCt < 1:1096892/57‑1 (MQ=255) aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg > 1:1786985/1‑35 (MQ=255) aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCggg > 1:2287539/1‑63 (MQ=255) aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTg < 1:1107964/70‑1 (MQ=255) aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGAGGGCGATCTGt > 1:2310319/1‑71 (MQ=255) aCGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGt < 1:644907/71‑1 (MQ=255) cGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTa > 1:624184/1‑71 (MQ=255) ccTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTa > 1:1566258/1‑69 (MQ=255) tCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGc > 1:1057033/1‑71 (MQ=255) | CAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGC > minE/817496‑817633 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |