Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 817,564 C→T L113L (CTC→CTT prfA → peptide chain release factor RF‑1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE817,5640CT95.7% 140.9 / ‑1.2 47L113L (CTC→CTTprfApeptide chain release factor RF‑1
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (23/22);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

CAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGC  >  minE/817496‑817633
                                                                    |                                                                     
cAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGa                                                                     <  1:968161/71‑1 (MQ=255)
cAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGa                                                                     <  1:1538685/71‑1 (MQ=255)
   cTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGt                                                                  >  1:21663/1‑71 (MQ=255)
      gAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc                                                                 <  1:832632/69‑1 (MQ=255)
              aTTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGaa                                                       >  1:556705/1‑71 (MQ=255)
              aTTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGaa                                                       >  1:229676/1‑71 (MQ=255)
               ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc                                                      <  1:2322269/71‑1 (MQ=255)
               ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc                                                      >  1:711993/1‑71 (MQ=255)
               ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc                                                      >  1:1714820/1‑71 (MQ=255)
               ttACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGaa                                                                    <  1:831734/57‑1 (MQ=255)
                 aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAcc                                                     >  1:956262/1‑70 (MQ=255)
                 aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg                                                    >  1:2419422/1‑71 (MQ=255)
                 aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg                                                    >  1:2208722/1‑71 (MQ=255)
                 aCAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg                                                    >  1:1205817/1‑71 (MQ=255)
                     gTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGcc                                                           >  1:1825407/1‑60 (MQ=255)
                     gTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcgg                                                   <  1:791117/68‑1 (MQ=255)
                     gTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg                                                    <  1:1323525/67‑1 (MQ=255)
                      ttCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCggcggc                                               <  1:696453/71‑1 (MQ=255)
                          gTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc                                                                 <  1:579702/49‑1 (MQ=255)
                          gTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc                                                                 <  1:1254546/49‑1 (MQ=255)
                           ttACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGcgacga                                          <  1:949029/71‑1 (MQ=255)
                             aCTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGaa                                         >  1:626153/1‑70 (MQ=255)
                              cTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAg                                        <  1:243170/70‑1 (MQ=255)
                                gCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGt                                                                  <  1:1556713/42‑1 (MQ=255)
                                 ccAAAAGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAAc                                                      >  1:1794142/1‑53 (MQ=255)
                                    aaaGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAgcgg                                     <  1:1787157/67‑1 (MQ=255)
                                      aGATCCTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg                                                    >  1:1227636/1‑50 (MQ=255)
                                       gATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc                                                                 >  1:2049883/1‑36 (MQ=255)
                                       gATCCTGATGACGAACGTAACGCCTTCCTTGAAGTc                                                                 >  1:1395202/1‑36 (MQ=255)
                                           cTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTcgcg                          <  1:2406752/71‑1 (MQ=255)
                                           cTGATGACGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTcgc                           <  1:1971296/70‑1 (MQ=255)
                                              atgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcggc                                                  >  1:2105485/1‑44 (MQ=255)
                                              atgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGc                       >  1:1777498/1‑71 (MQ=255)
                                               tgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCgg                         <  1:2341330/68‑1 (MQ=255)
                                               tgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGc                       >  1:1831853/1‑70 (MQ=255)
                                               tgaCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCg                      <  1:658994/71‑1 (MQ=255)
                                                 aCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCg                      <  1:1697700/69‑1 (MQ=255)
                                                 aCGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGa                     <  1:1768541/70‑1 (MQ=255)
                                                  cGAACGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCt                                 <  1:1096892/57‑1 (MQ=255)
                                                     aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACcg                                                    >  1:1786985/1‑35 (MQ=255)
                                                     aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCggg                        >  1:2287539/1‑63 (MQ=255)
                                                     aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTg                 <  1:1107964/70‑1 (MQ=255)
                                                     aCGTAACGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGAGGGCGATCTGt                >  1:2310319/1‑71 (MQ=255)
                                                          aCGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGt           <  1:644907/71‑1 (MQ=255)
                                                           cGCCTTCCTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTa          >  1:624184/1‑71 (MQ=255)
                                                                 ccTTGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTa      >  1:1566258/1‑69 (MQ=255)
                                                                   tCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGc  >  1:1057033/1‑71 (MQ=255)
                                                                    |                                                                     
CAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGC  >  minE/817496‑817633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: