| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 894,732 | C→T | D445N (GAC→AAC) | sapA ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 894,732 | 0 | C | T | 97.3% | 112.3 / ‑3.6 | 37 | D445N (GAC→AAC) | sapA | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (23/13); total (24/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAA > minE/894670‑894792 | gTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGt < 1:1823150/71‑1 (MQ=255) gggCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTa > 1:101801/1‑71 (MQ=255) cGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTg > 1:770548/1‑71 (MQ=255) cTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGcc > 1:1633567/1‑70 (MQ=255) tGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCggg > 1:2389458/1‑51 (MQ=255) cGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAAc < 1:2017611/71‑1 (MQ=255) gaatgaatTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTTCTGTCCGTCGCCCAAcc > 1:1355276/1‑71 (MQ=255) gaatgaatTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAAcc > 1:891884/1‑71 (MQ=255) gaatgaatTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAAc < 1:122722/70‑1 (MQ=255) gaatTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGa > 1:1399273/1‑70 (MQ=255) atTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATaa < 1:1688502/71‑1 (MQ=255) ttGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAAc > 1:754014/1‑71 (MQ=255) ccGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGt < 1:1371309/70‑1 (MQ=255) ccGCGCAGCTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGt < 1:226344/70‑1 (MQ=255) cTCAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAg < 1:875899/65‑1 (MQ=255) cAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAAc > 1:439373/1‑58 (MQ=255) cAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGAt > 1:1472812/1‑65 (MQ=255) cAGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATgg > 1:1382149/1‑70 (MQ=255) aGTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGc < 1:2407607/70‑1 (MQ=255) gTAACGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATaa < 1:1785007/55‑1 (MQ=255) aaCGGACGGAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACg > 1:1203708/1‑55 (MQ=255) acggacggAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCa > 1:1080503/1‑43 (MQ=255) acggacggAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCata > 1:2288645/1‑71 (MQ=255) acggacggAAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCata > 1:52342/1‑71 (MQ=255) gacggaAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCaa > 1:1667854/1‑41 (MQ=255) acggaAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCa > 1:587848/1‑71 (MQ=255) cggaAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATcc > 1:2344309/1‑69 (MQ=255) cggaAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATcc > 1:74881/1‑69 (MQ=255) cggaAGAAACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATcc > 1:1845819/1‑69 (MQ=255) gaagaaACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCa < 1:575283/71‑1 (MQ=255) gaagaaACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAAAGTCAGAt > 1:620631/1‑53 (MQ=39) aagaaACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATcc > 1:1978932/1‑66 (MQ=255) aagaaACTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCaa < 1:436536/71‑1 (MQ=255) aaCTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGa > 1:1423574/1‑47 (MQ=255) aaCTGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCt > 1:1324148/1‑55 (MQ=255) tGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAAc < 1:2007258/38‑1 (MQ=255) tGTTCGGGTCGTTACTGTCCGTCGCCCAACCGGATAAc < 1:741799/38‑1 (MQ=255) | GTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATCATGGCTCATATCCATCAA > minE/894670‑894792 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |