| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,036,505 | G→A | 100% | A632A (GCG→GCA) | rsxC → | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,036,505 | 0 | G | A | 98.2% | 121.3 / ‑5.5 | 48 | A632A (GCG→GCA) | rsxC | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (29/18); minor base C (0/1); total (29/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCG > minE/1036438‑1036572 | cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg > 1:2997194/1‑70 (MQ=25)cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg < 1:3306334/70‑1 (MQ=25) ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt > 1:626171/1‑71 (MQ=21) cAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg > 1:1691315/1‑71 (MQ=21) aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacgc > 1:1957299/1‑62 (MQ=25) aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa > 1:1563277/1‑64 (MQ=25) agaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCAGTGCCAAAGCACGCAAGCTg > 1:3191490/1‑67 (MQ=255) gaaCAGATCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa > 1:2760775/1‑69 (MQ=255) gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc < 1:1744277/71‑1 (MQ=14) aaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg > 1:3762797/1‑71 (MQ=25) aaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg > 1:425037/1‑71 (MQ=25) aaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg > 1:1286372/1‑71 (MQ=25) gccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg > 1:3145908/1‑70 (MQ=255) ccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc < 1:4229799/62‑1 (MQ=14) cgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:1240979/71‑1 (MQ=255) gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaac < 1:4159477/70‑1 (MQ=255) gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca < 1:2915994/71‑1 (MQ=255) cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg < 1:3642724/71‑1 (MQ=255) gCTATTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:3274149/61‑1 (MQ=255) gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg < 1:664645/70‑1 (MQ=255) gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg < 1:2785947/70‑1 (MQ=255) gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg < 1:3968991/70‑1 (MQ=255) tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt > 1:305658/1‑70 (MQ=255) tGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc > 1:1531241/1‑71 (MQ=255) tGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc > 1:1788886/1‑71 (MQ=255) gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:2604101/1‑53 (MQ=255) gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg > 1:1667186/1‑71 (MQ=255) gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg > 1:1227596/1‑71 (MQ=255) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa < 1:3525823/64‑1 (MQ=255) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa < 1:917563/64‑1 (MQ=255) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa < 1:2316622/64‑1 (MQ=255) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg < 1:2613876/70‑1 (MQ=255) cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATc > 1:2107620/1‑64 (MQ=255) cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATc > 1:1248955/1‑64 (MQ=255) cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa < 1:1738119/71‑1 (MQ=255) gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCTCaaa < 1:2964298/71‑1 (MQ=255) gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa < 1:425204/71‑1 (MQ=255) tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa < 1:4242266/70‑1 (MQ=255) ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:3118580/1‑46 (MQ=255) ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:2600145/1‑46 (MQ=255) ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:2723222/1‑46 (MQ=255) ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:2304922/1‑46 (MQ=255) ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:3749038/1‑46 (MQ=255) ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:3688866/1‑46 (MQ=255) ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:3633999/1‑46 (MQ=255) ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:1823360/1‑46 (MQ=255) cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa < 1:1683490/66‑1 (MQ=255) aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgc > 1:1704835/1‑71 (MQ=255) aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAg > 1:653030/1‑66 (MQ=255) aaGCACACAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAg > 1:571755/1‑66 (MQ=255) aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGccgccg > 1:2915859/1‑71 (MQ=255) | CGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCG > minE/1036438‑1036572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |