| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,221,883 | A→T | 6.0% | K153I (AAA→ATA) | yebT → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,221,883 | 0 | A | T | 6.0% | 290.3 / 8.3 | 99 | K153I (AAA→ATA) | yebT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (51/42); new base T (2/4); total (53/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
AACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGC > minE/1221813‑1221950 | aaCTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGaa < 1:3085703/71‑1 (MQ=255) cTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGaaa > 1:4257227/1‑70 (MQ=255) cTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAat < 1:2595770/71‑1 (MQ=255) cTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAat < 1:3931379/71‑1 (MQ=255) cTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAat < 1:351015/71‑1 (MQ=255) tatCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAatat > 1:2924238/1‑70 (MQ=255) tatCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATc < 1:2798219/71‑1 (MQ=255) tatCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATc > 1:1528017/1‑71 (MQ=255) gCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTg > 1:70409/1‑71 (MQ=255) atgatgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAGCCGATATTTCGGTTggg < 1:250044/71‑2 (MQ=38) atgatgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAGCCGATATTTCGGTTggg < 1:3955505/71‑2 (MQ=38) atgatgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAGCCGATATTTCGGTTggg < 1:2652691/71‑2 (MQ=38) atgatgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAGCCGATATTTCGGTTggg < 1:2802799/71‑2 (MQ=38) tgatgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGa > 1:2012182/1‑70 (MQ=255) gatgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAGCCGATATTTCGGTTgggta > 1:3587952/1‑68 (MQ=37) gatgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAGCCGATATTTCGGTTgggta > 1:906849/1‑68 (MQ=37) atgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACaa > 1:2725512/1‑71 (MQ=255) atgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACaa > 1:3852980/1‑71 (MQ=255) atgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACaa > 1:802471/1‑71 (MQ=255) atgCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACaa > 1:1029857/1‑71 (MQ=255) gtaaaggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGAt > 1:4177585/1‑71 (MQ=255) taaaggtaaagAGCAGTATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCg < 1:4320184/68‑1 (MQ=255) taaaggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAat < 1:2924015/50‑1 (MQ=255) taaaggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCg < 1:3559872/68‑1 (MQ=255) taaaggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCg < 1:3412172/68‑1 (MQ=255) taaaggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATc > 1:2849333/1‑71 (MQ=255) aaggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCt > 1:4073706/1‑55 (MQ=255) aaggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCt > 1:3292777/1‑55 (MQ=255) aaggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCt > 1:3794254/1‑55 (MQ=255) aaggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCt < 1:4350618/70‑1 (MQ=255) aggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtga > 1:3518189/1‑71 (MQ=255) aggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtga > 1:1707162/1‑71 (MQ=255) aggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtga > 1:3089539/1‑71 (MQ=255) aggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtga > 1:3657989/1‑71 (MQ=255) ggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGa > 1:2567144/1‑56 (MQ=255) ggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGa > 1:1809656/1‑56 (MQ=255) ggtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtgat < 1:3418502/71‑1 (MQ=255) gtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGaaa > 1:440773/1‑44 (MQ=255) gtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGaaa > 1:1159695/1‑44 (MQ=255) gtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGaaa > 1:2771463/1‑44 (MQ=255) gtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTg < 1:4046307/53‑1 (MQ=255) gtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTg < 1:859358/53‑1 (MQ=255) gtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTg < 1:2656646/53‑1 (MQ=255) gtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACaa > 1:2414956/1‑58 (MQ=255) gtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtgatg < 1:452570/71‑1 (MQ=255) gtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtgatg < 1:1226216/71‑1 (MQ=255) gtaaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtgat > 1:4003162/1‑70 (MQ=255) taaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGAGATCtgatg > 1:2503251/1‑70 (MQ=255) aaagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGAt > 1:3519554/1‑63 (MQ=255) aagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGAt > 1:3959734/1‑62 (MQ=255) aagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGAt > 1:3108247/1‑62 (MQ=255) aagAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATc > 1:3429980/1‑71 (MQ=255) agagCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCg < 1:2750500/45‑1 (MQ=255) agagCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCg < 1:1423413/45‑1 (MQ=255) agagCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCg < 1:868985/45‑1 (MQ=255) gCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCAc < 1:2187188/70‑1 (MQ=255) cAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCt < 1:1151494/71‑1 (MQ=255) cAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCt < 1:1546748/71‑1 (MQ=255) aGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTg > 1:3115701/1‑71 (MQ=255) ggATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGc < 1:3742953/71‑1 (MQ=255) aTCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGc < 1:2464709/69‑1 (MQ=255) tttGTCGCACTCGATACCCAACCGAAATATCGGCTGGAc < 1:1780079/39‑1 (MQ=255) tttGTCGCACTCGATACCCAACCGAAATATCGGCTGGAc < 1:806404/39‑1 (MQ=255) tGTCGCACTCGATACCCAACCGAAATATCGGCTGGACaa > 1:1275829/1‑39 (MQ=255) tGTCGCACTCGATACCCAACCGAAATATCGGCTGGACaa > 1:3476280/1‑39 (MQ=255) tGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGAt > 1:1969534/1‑46 (MQ=255) tGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGccc < 1:1301739/68‑1 (MQ=255) gCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGAtct < 1:1955496/71‑1 (MQ=255) cACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGAtctc > 1:3299012/1‑71 (MQ=255) cACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGAtctc > 1:191557/1‑71 (MQ=255) cACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGAtctc < 1:1100457/71‑1 (MQ=255) cTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCgg > 1:2097516/1‑71 (MQ=255) cTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCgg < 1:2811734/71‑1 (MQ=255) cTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCgg > 1:77109/1‑71 (MQ=255) tCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtgat < 1:3575275/43‑1 (MQ=255) tCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtgat < 1:3488793/43‑1 (MQ=255) cGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCAc > 1:2631649/1‑49 (MQ=255) cGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGtt > 1:2608283/1‑71 (MQ=255) gATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAg > 1:2740496/1‑55 (MQ=255) gATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTc > 1:1940407/1‑71 (MQ=255) gATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTc > 1:4136526/1‑71 (MQ=255) gATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTc > 1:1348553/1‑71 (MQ=255) tACCCAACCGAAATATCGGCTGGACAATGGCGATCTgatgat < 1:3089195/42‑1 (MQ=255) tACCCAACCGAAATATCGGCTGGACAATGGCGATCTgatgat < 1:1265361/42‑1 (MQ=255) tACCCAACCGAAATATCGGCTGGACAATGGCGATCTgatgat < 1:2289012/42‑1 (MQ=255) tACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCa > 1:1649786/1‑51 (MQ=255) tACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCa > 1:741907/1‑51 (MQ=255) cccAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCt > 1:614977/1‑70 (MQ=255) cccAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTg < 1:4404829/71‑1 (MQ=255) ccAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGAtct < 1:2667694/60‑1 (MQ=255) ccAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGAtct < 1:1911608/60‑1 (MQ=255) cAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGaa > 1:1774182/1‑71 (MQ=255) cAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGaa > 1:2272879/1‑71 (MQ=255) cAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGaa > 1:2990037/1‑71 (MQ=255) cAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGaa > 1:3159878/1‑71 (MQ=255) aaCCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAAc < 1:2911884/71‑1 (MQ=255) aCCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGaa > 1:3417214/1‑69 (MQ=255) aCCGAAATATAGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGaa > 1:1017712/1‑69 (MQ=255) ccGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAg < 1:814123/71‑1 (MQ=255) cGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGc < 1:495221/71‑1 (MQ=255) | AACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGC > minE/1221813‑1221950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |