| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,256,819 | A→G | 7.6% | G73G (GGT→GGC) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,256,819 | 0 | A | G | 7.6% | 187.7 / 6.8 | 66 | G73G (GGT→GGC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base A (30/31); new base G (3/2); total (33/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01 | |||||||||||
CTTTAGTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGT > minE/2256749‑2256888 | cTTTAGTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTa > 1:956842/1‑71 (MQ=255) tAGTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTttacttac < 1:2455670/69‑1 (MQ=255) tAGTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTa > 1:3504351/1‑68 (MQ=255) tAGTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTa > 1:1893311/1‑68 (MQ=255) tAGTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTa > 1:2355965/1‑68 (MQ=255) tAGTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTAc < 1:2859699/69‑1 (MQ=255) tAGTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCg > 1:833099/1‑71 (MQ=255) tAGTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCg > 1:2964687/1‑71 (MQ=255) tAGTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACATACCg > 1:1261062/1‑71 (MQ=255) gTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGca < 1:4183438/71‑1 (MQ=255) tCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGcac < 1:2688547/71‑1 (MQ=255) tGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAg > 1:3574906/1‑71 (MQ=255) ggTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAg < 1:1342881/69‑1 (MQ=255) cACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACt > 1:2773609/1‑71 (MQ=255) cACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACt > 1:465325/1‑71 (MQ=255) aCTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTc > 1:4043579/1‑71 (MQ=255) cTTCGACGCCGCACTTTTCGCAGATCACGCCGCGGTGCTTCAGGCGCTTGTACTTGCCGCACAGGCATTCg > 1:3602722/1‑71 (MQ=255) cTTCGACGCCGCACTTTTCGCAGATCACGCCGCGGTGCTTCAGGCGCTTGTACTTGCCGCACAGGCATTCg > 1:4149514/1‑71 (MQ=255) ttCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGca < 1:4205750/60‑1 (MQ=255) ttCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGca < 1:292678/60‑1 (MQ=255) ttCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGca < 1:2852610/60‑1 (MQ=255) gccgcACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCtt < 1:3641170/71‑1 (MQ=255) gcACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa < 1:721655/71‑1 (MQ=255) gcACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa < 1:267855/71‑1 (MQ=255) gcACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa < 1:2852369/71‑1 (MQ=255) cACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTAc < 1:155797/71‑1 (MQ=255) aCTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTAcc < 1:3956610/71‑1 (MQ=255) cTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCttt < 1:4324115/66‑1 (MQ=255) ttCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGcac < 1:2212390/48‑1 (MQ=255) tctcACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGc > 1:1482223/1‑45 (MQ=255) tctcACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:634881/1‑66 (MQ=255) tctcACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:3535335/1‑66 (MQ=255) ctcACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCgga > 1:2215632/1‑69 (MQ=255) ctcACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCgg < 1:3844695/69‑1 (MQ=255) acaGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTAc > 1:2744608/1‑63 (MQ=255) acaGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCa > 1:1594818/1‑70 (MQ=255) caGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGccc < 1:235584/68‑1 (MQ=255) caGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCaaa < 1:4395260/71‑1 (MQ=255) aGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:2945820/1‑60 (MQ=255) aGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:240790/1‑60 (MQ=255) aGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCaaa < 1:1589896/70‑1 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTAc > 1:3654770/1‑35 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCa > 1:2587188/1‑67 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCa > 1:2564452/1‑67 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCa > 1:39560/1‑67 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGa < 1:1145780/71‑1 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGa < 1:2041196/71‑1 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGa < 1:2046605/71‑1 (MQ=255) aTGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:963957/1‑58 (MQ=255) aTGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:2376058/1‑58 (MQ=255) cACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCTTAATCTTTTACCGGCCCAAAGATACGGgcg < 1:1673729/71‑1 (MQ=255) cACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGgcg < 1:1521080/71‑1 (MQ=255) aCGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTa < 1:3575386/42‑1 (MQ=255) cGGTGCTTCAGGCGCTTGTACTTGCCGCACAGGCATTCGTAGTCCTTGATCGGGCCAAAGATCTTGGCGCa < 1:1482452/71‑1 (MQ=255) ggTGCTTCAGGCGCTTGTACTTGCCGCACAGGCATTCGTAGTCCTTGATCGGGCCAAAGATCTTGGCGCa < 1:3895856/70‑1 (MQ=255) tgtTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaa < 1:3884487/71‑1 (MQ=255) tgtTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaa < 1:891174/71‑1 (MQ=255) tttCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaaaa < 1:2673864/71‑1 (MQ=255) tttCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaaa < 1:4409524/70‑1 (MQ=255) cAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATa < 1:4054929/55‑1 (MQ=255) cAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATa < 1:1226017/55‑1 (MQ=255) ggCGCTTGTACTTGCCGCACAGGCACTCGTAGTCCTTCACCGGGCCGAAGATCTTGGCGCAGAACAGGCCg > 1:547731/1‑71 (MQ=255) ggCGCTTGTACTTACCGCACAGGCTCTCGTAATCTTTTACCGGCCCAAAGATAc > 1:298424/1‑54 (MQ=255) tGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGt > 1:1622404/1‑71 (MQ=255) gTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCa > 1:258386/1‑67 (MQ=255) gTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGtt > 1:739846/1‑71 (MQ=255) gTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGt > 1:3443924/1‑70 (MQ=255) aCTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCAc > 1:3716180/1‑66 (MQ=255) aCTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCAc > 1:1136026/1‑66 (MQ=255) ttACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTgg > 1:3562054/1‑71 (MQ=255) tACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGt > 1:2436464/1‑71 (MQ=255) | CTTTAGTCTGGGTCACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGT > minE/2256749‑2256888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |