| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,256,831 | C→T | 6.0% | E69E (GAG→GAA) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,256,831 | 0 | C | T | 6.0% | 219.7 / 3.9 | 66 | E69E (GAG→GAA) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base C (29/33); new base T (2/2); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
CACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGG > minE/2256762‑2256900 | cACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACt > 1:465325/1‑71 (MQ=255)cACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACt > 1:2773609/1‑71 (MQ=255) aCTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTc > 1:4043579/1‑71 (MQ=255) cTTCGACGCCGCACTTTTCGCAGATCACGCCGCGGTGCTTCAGGCGCTTGTACTTGCCGCACAGGCATTCg > 1:4149514/1‑71 (MQ=255) cTTCGACGCCGCACTTTTCGCAGATCACGCCGCGGTGCTTCAGGCGCTTGTACTTGCCGCACAGGCATTCg > 1:3602722/1‑71 (MQ=255) gccgcACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCtt < 1:3641170/71‑1 (MQ=255) gcACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa < 1:721655/71‑1 (MQ=255) gcACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa < 1:267855/71‑1 (MQ=255) gcACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa < 1:2852369/71‑1 (MQ=255) cACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTAc < 1:155797/71‑1 (MQ=255) aCTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTAcc < 1:3956610/71‑1 (MQ=255) cTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCttt < 1:4324115/66‑1 (MQ=255) tctcACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:634881/1‑66 (MQ=255) tctcACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:3535335/1‑66 (MQ=255) ctcACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCgga > 1:2215632/1‑69 (MQ=255) ctcACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCgg < 1:3844695/69‑1 (MQ=255) acaGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTAc > 1:2744608/1‑63 (MQ=255) acaGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCa > 1:1594818/1‑70 (MQ=255) caGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGccc < 1:235584/68‑1 (MQ=255) caGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCaaa < 1:4395260/71‑1 (MQ=255) aGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:2945820/1‑60 (MQ=255) aGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:240790/1‑60 (MQ=255) aGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCaaa < 1:1589896/70‑1 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCa > 1:39560/1‑67 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCa > 1:2564452/1‑67 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCa > 1:2587188/1‑67 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGa < 1:1145780/71‑1 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGa < 1:2046605/71‑1 (MQ=255) gATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGa < 1:2041196/71‑1 (MQ=255) aTGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:963957/1‑58 (MQ=255) aTGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTa > 1:2376058/1‑58 (MQ=255) cACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCTTAATCTTTTACCGGCCCAAAGATACGGgcg < 1:1673729/71‑1 (MQ=255) cACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGgcg < 1:1521080/71‑1 (MQ=255) aCGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTa < 1:3575386/42‑1 (MQ=255) cGGTGCTTCAGGCGCTTGTACTTGCCGCACAGGCATTCGTAGTCCTTGATCGGGCCAAAGATCTTGGCGCa < 1:1482452/71‑1 (MQ=255) ggTGCTTCAGGCGCTTGTACTTGCCGCACAGGCATTCGTAGTCCTTGATCGGGCCAAAGATCTTGGCGCa < 1:3895856/70‑1 (MQ=255) tgtTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaa < 1:891174/71‑1 (MQ=255) tgtTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaa < 1:3884487/71‑1 (MQ=255) tttCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaaaa < 1:2673864/71‑1 (MQ=255) tttCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaaa < 1:4409524/70‑1 (MQ=255) cAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATa < 1:4054929/55‑1 (MQ=255) cAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATa < 1:1226017/55‑1 (MQ=255) ggCGCTTGTACTTGCCGCACAGGCACTCGTAGTCCTTCACCGGGCCGAAGATCTTGGCGCAGAACAGGCCg > 1:547731/1‑71 (MQ=255) ggCGCTTGTACTTACCGCACAGGCTCTCGTAATCTTTTACCGGCCCAAAGATAc > 1:298424/1‑54 (MQ=255) tGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGt > 1:1622404/1‑71 (MQ=255) gTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCa > 1:258386/1‑67 (MQ=255) gTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGtt > 1:739846/1‑71 (MQ=255) gTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGt > 1:3443924/1‑70 (MQ=255) aCTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCAc > 1:3716180/1‑66 (MQ=255) aCTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCAc > 1:1136026/1‑66 (MQ=255) ttACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTgg > 1:3562054/1‑71 (MQ=255) tACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGt > 1:2436464/1‑71 (MQ=255) ccGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACTTTCTGGtt < 1:2982129/70‑1 (MQ=255) ccGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGtt > 1:1429869/1‑70 (MQ=255) gCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATAc > 1:4226562/1‑38 (MQ=255) gCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGtt > 1:265228/1‑62 (MQ=255) gCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGtt > 1:2468014/1‑68 (MQ=255) cacaGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGt < 1:445381/66‑1 (MQ=255) cacaGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGt < 1:1637287/66‑1 (MQ=255) cacaGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGaa < 1:123186/71‑1 (MQ=255) ggCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTa < 1:1505071/71‑1 (MQ=255) ggCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTa < 1:395793/71‑1 (MQ=255) gCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTAc < 1:3306271/71‑1 (MQ=255) cACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGcc < 1:1704308/47‑1 (MQ=255) aCTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACgg < 1:1487578/71‑1 (MQ=255) aCTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACg < 1:4181078/70‑1 (MQ=255) | CACTTCAACGCCGCACTTCTCACAGATGACGCCACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGG > minE/2256762‑2256900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |