| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,256,859 | C→T | 5.8% | R60H (CGT→CAT) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,256,859 | 0 | C | T | 5.8% | 231.3 / 2.1 | 68 | R60H (CGT→CAT) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base C (31/33); new base T (2/2); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTC > minE/2256794‑2256929 | cACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCTTAATCTTTTACCGGCCCAAAGATACGGgcg < 1:1673729/71‑1 (MQ=255)cACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGgcg < 1:1521080/71‑1 (MQ=255) cGGTGCTTCAGGCGCTTGTACTTGCCGCACAGGCATTCGTAGTCCTTGATCGGGCCAAAGATCTTGGCGCa < 1:1482452/71‑1 (MQ=255) ggTGCTTCAGGCGCTTGTACTTGCCGCACAGGCATTCGTAGTCCTTGATCGGGCCAAAGATCTTGGCGCa < 1:3895856/70‑1 (MQ=255) tgtTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaa < 1:891174/71‑1 (MQ=255) tgtTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaa < 1:3884487/71‑1 (MQ=255) tttCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaaaa < 1:2673864/71‑1 (MQ=255) tttCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaaa < 1:4409524/70‑1 (MQ=255) ggCGCTTGTACTTGCCGCACAGGCACTCGTAGTCCTTCACCGGGCCGAAGATCTTGGCGCAGAACAGGCCg > 1:547731/1‑71 (MQ=255) ggCGCTTGTACTTACCGCACAGGCTCTCGTAATCTTTTACCGGCCCAAAGATAc > 1:298424/1‑54 (MQ=255) tGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGt > 1:1622404/1‑71 (MQ=255) gTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCa > 1:258386/1‑67 (MQ=255) gTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGtt > 1:739846/1‑71 (MQ=255) gTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGt > 1:3443924/1‑70 (MQ=255) aCTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCAc > 1:1136026/1‑66 (MQ=255) aCTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCAc > 1:3716180/1‑66 (MQ=255) ttACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTgg > 1:3562054/1‑71 (MQ=255) tACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGt > 1:2436464/1‑71 (MQ=255) ccGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACTTTCTGGtt < 1:2982129/70‑1 (MQ=255) ccGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGtt > 1:1429869/1‑70 (MQ=255) gCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATAc > 1:4226562/1‑38 (MQ=255) gCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGtt > 1:265228/1‑62 (MQ=255) gCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGtt > 1:2468014/1‑68 (MQ=255) cacaGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGt < 1:1637287/66‑1 (MQ=255) cacaGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGt < 1:445381/66‑1 (MQ=255) cacaGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGaa < 1:123186/71‑1 (MQ=255) ggCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTa < 1:395793/71‑1 (MQ=255) ggCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTa < 1:1505071/71‑1 (MQ=255) gCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTAc < 1:3306271/71‑1 (MQ=255) cACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGcc < 1:1704308/47‑1 (MQ=255) aCTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACgg < 1:1487578/71‑1 (MQ=255) aCTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACg < 1:4181078/70‑1 (MQ=255) tCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTa < 1:2777307/66‑1 (MQ=255) cGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGaaaa > 1:743604/1‑39 (MQ=255) gTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAg < 1:3275453/70‑1 (MQ=255) gTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAg < 1:1440229/70‑1 (MQ=255) gTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGt < 1:2014580/71‑1 (MQ=255) tAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACg < 1:3996170/65‑1 (MQ=255) tAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGtt < 1:2354691/71‑1 (MQ=255) tAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGtt < 1:3327321/71‑1 (MQ=255) aaTCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAg > 1:3482977/1‑68 (MQ=255) aTCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGcc > 1:1659029/1‑39 (MQ=255) aTCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGa > 1:1257734/1‑71 (MQ=255) ttttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGaa > 1:2106230/1‑54 (MQ=255) ttttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGaa > 1:4421492/1‑54 (MQ=255) ttttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATg > 1:936954/1‑70 (MQ=255) tttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCAc > 1:2845458/1‑40 (MQ=255) ttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTGTGGTTTGAACGTACGGTa > 1:2938719/1‑61 (MQ=255) ttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGaa > 1:1694250/1‑52 (MQ=255) ttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTa > 1:3225976/1‑61 (MQ=255) ttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTa > 1:3060998/1‑61 (MQ=255) ttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTa > 1:2447908/1‑61 (MQ=255) ttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGAt < 1:1960950/67‑1 (MQ=255) ttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGAt < 1:3284622/67‑1 (MQ=255) ttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGAt < 1:4133918/67‑1 (MQ=255) ttACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGtt > 1:1802535/1‑71 (MQ=255) tACCGGCCCAAAGATATGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGttt > 1:284008/1‑71 (MQ=255) tACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGttt > 1:2535651/1‑71 (MQ=255) tACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGttt > 1:2960344/1‑71 (MQ=255) tACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGtt > 1:737328/1‑70 (MQ=255) aCCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGtt < 1:320276/69‑1 (MQ=255) cGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATg > 1:928874/1‑64 (MQ=255) cAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCttt < 1:2863439/71‑1 (MQ=255) aaGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTa < 1:4282311/45‑1 (MQ=255) aaGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCttttt < 1:4385838/71‑1 (MQ=255) aGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCttttt > 1:2844118/1‑70 (MQ=255) aGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTa < 1:4342694/71‑1 (MQ=255) gATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTaa < 1:38990/71‑1 (MQ=255) aTACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAAc > 1:1584575/1‑71 (MQ=255) tACTGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACt < 1:146928/71‑1 (MQ=255) cGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACg > 1:1839761/1‑37 (MQ=255) cGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTc < 1:3720016/71‑1 (MQ=255) cGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTATCCGGCTTTTTAACTTc < 1:299158/71‑1 (MQ=255) | CACGGTGTTTCAGGCGCTTGTACTTACCGCACAGGCACTCGTAATCTTTTACCGGCCCAAAGATACGGGCGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTC > minE/2256794‑2256929 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |