| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,518,841 | A→T | 7.2% | T78T (ACT→ACA) | spoT ← | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,518,841 | 0 | A | T | 7.2% | 238.1 / 9.7 | 83 | T78T (ACT→ACA) | spoT | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (53/24); new base T (4/2); total (57/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
CGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAG > minE/2518772‑2518909 | cGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAg > 1:3815817/1‑71 (MQ=255)cGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAg > 1:3425813/1‑71 (MQ=255) gACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGt < 1:3278142/71‑1 (MQ=255) gACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGt < 1:4274837/71‑1 (MQ=255) ccTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTc > 1:3362916/1‑70 (MQ=255) ccTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTc > 1:2871096/1‑70 (MQ=255) tctACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCa < 1:1580948/69‑1 (MQ=255) tctACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAAt > 1:3201122/1‑71 (MQ=255) tACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAAt < 1:934720/69‑1 (MQ=255) aCCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCAc > 1:3060684/1‑71 (MQ=255) aCCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCAc > 1:842526/1‑71 (MQ=255) ccAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATct < 1:2622718/69‑2 (MQ=255) ccAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCa < 1:3798712/69‑1 (MQ=255) cAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:2977191/1‑71 (MQ=255) aGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCaa > 1:1465233/1‑64 (MQ=255) aGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCaa > 1:3358767/1‑64 (MQ=255) aGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:2964796/1‑70 (MQ=255) gCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCa < 1:1435286/71‑1 (MQ=255) gCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCa < 1:2189596/71‑1 (MQ=255) tCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATg > 1:3332155/1‑71 (MQ=255) cGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATg > 1:2715722/1‑70 (MQ=255) ggCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATgca < 1:605792/71‑1 (MQ=255) cGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATgcag > 1:2694040/1‑70 (MQ=255) cGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATgca > 1:3796510/1‑66 (MQ=255) cGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCagcagc > 1:52649/1‑71 (MQ=255) cGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCagcagc > 1:828202/1‑71 (MQ=255) gCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAgcg > 1:940297/1‑71 (MQ=255) cTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAAt > 1:3726286/1‑53 (MQ=255) cTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAAt > 1:955951/1‑53 (MQ=255) cTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAAt > 1:109650/1‑53 (MQ=255) cTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAAt > 1:3283891/1‑53 (MQ=255) tttACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCa < 1:192008/49‑1 (MQ=255) tttACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCa < 1:1225846/49‑1 (MQ=255) tACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGa > 1:3918876/1‑38 (MQ=255) ccAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCAt > 1:3928624/1‑71 (MQ=255) ccAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCAt > 1:4001146/1‑71 (MQ=255) ccAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCAt > 1:1009818/1‑71 (MQ=255) cTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTc > 1:271569/1‑36 (MQ=255) cTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTc > 1:1389926/1‑36 (MQ=255) tCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTc > 1:1291767/1‑68 (MQ=255) ccATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAg > 1:355024/1‑71 (MQ=255) cATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACg > 1:4266451/1‑37 (MQ=255) atatCCTGGTATGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTc < 1:2124787/71‑1 (MQ=255) atatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:4058352/1‑37 (MQ=255) atatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:436797/1‑37 (MQ=255) atatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:1637602/1‑37 (MQ=255) atatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:3118645/1‑37 (MQ=255) atatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:2769226/1‑37 (MQ=255) tatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTc > 1:3261600/1‑70 (MQ=255) tatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTc > 1:3861253/1‑70 (MQ=255) tCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATgcagca > 1:728838/1‑43 (MQ=255) tCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATgca > 1:2172637/1‑40 (MQ=255) tCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGcagcag > 1:2469247/1‑44 (MQ=255) tCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGcagcag > 1:1308013/1‑44 (MQ=255) ccTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGt > 1:492457/1‑71 (MQ=255) ccTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGt > 1:471984/1‑71 (MQ=255) ccTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGt > 1:633174/1‑71 (MQ=255) ccTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGt > 1:3383147/1‑71 (MQ=255) ccTGGTAGGTGGAGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGt > 1:2738902/1‑71 (MQ=255) cTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGtt < 1:3573452/71‑1 (MQ=255) cTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGtt < 1:945360/71‑1 (MQ=255) tGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCg < 1:3272235/55‑1 (MQ=255) tGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCg < 1:2022786/55‑1 (MQ=255) tGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCg < 1:1956127/55‑1 (MQ=255) tGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGtt > 1:3466994/1‑70 (MQ=255) gTAGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCg < 1:3813527/53‑1 (MQ=39) gTAGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCg < 1:2014347/53‑1 (MQ=39) gTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGttt < 1:3314495/69‑1 (MQ=255) gTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGttt < 1:4276813/69‑1 (MQ=255) gTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGttt < 1:2595588/69‑1 (MQ=255) gTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTc < 1:2848010/70‑1 (MQ=255) aGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCGtt > 1:3643209/1‑53 (MQ=39) aGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCGtt > 1:3331001/1‑53 (MQ=39) aGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCGtt > 1:3182362/1‑53 (MQ=39) aGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCGtt > 1:985662/1‑53 (MQ=39) aGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCAtc > 1:3493867/1‑71 (MQ=255) ggTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCAtct > 1:2268266/1‑71 (MQ=255) tGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGttt < 1:3811393/64‑1 (MQ=255) tGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGttt < 1:904977/64‑1 (MQ=255) ggCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCgg > 1:3122182/1‑71 (MQ=255) gCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCa > 1:716077/1‑64 (MQ=255) gCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCa > 1:2512466/1‑64 (MQ=255) gCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTAGAGTTTCa > 1:1914530/1‑64 (MQ=255) ggAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAg < 1:2001533/71‑1 (MQ=255) | CGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAG > minE/2518772‑2518909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |