| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,567,261 | G→A | 5.9% | intergenic (‑210/+20) | mtlD ← / ← mtlA | mannitol‑1‑phosphate dehydrogenase, NAD(P)‑binding/fused mannitol‑specific PTS enzyme IIABC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,567,261 | 0 | G | A | 5.9% | 412.3 / 10.3 | 118 | intergenic (‑210/+20) | mtlD/mtlA | mannitol‑1‑phosphate dehydrogenase, NAD(P)‑binding/fused mannitol‑specific PTS enzyme IIABC components |
| Reads supporting (aligned to +/- strand): ref base G (40/71); new base A (4/3); total (44/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
AACAAATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTGGTGGT > minE/2567191‑2567328 | aacaaATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAtg < 1:781704/71‑1 (MQ=255) acaaATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAtgt > 1:891699/1‑71 (MQ=255) aaaTTTTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTgg < 1:2956612/71‑1 (MQ=255) aaTTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGga < 1:3006938/71‑1 (MQ=255) tgtgATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGagag > 1:4409376/1‑71 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGt < 1:3590455/70‑1 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGt < 1:3242477/70‑1 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGt < 1:1176472/70‑1 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTg < 1:298036/71‑1 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCCTCTCCACATGGAGAAGGt < 1:3091785/70‑1 (MQ=21) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCCTCTCCACATGGAGAAGGt < 1:4439536/70‑1 (MQ=21) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCCTCTCCACATGGAGAAGGt < 1:3588592/70‑1 (MQ=21) tGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGt < 1:3330848/69‑1 (MQ=255) tGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTgg < 1:2445853/71‑1 (MQ=255) tGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTgg < 1:1884574/71‑1 (MQ=255) tGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTg < 1:428253/70‑1 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAtgtg < 1:1973435/59‑1 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAtgtg < 1:2793174/59‑1 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAtgtg < 1:4003552/59‑1 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTggg < 1:282487/70‑1 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGgga < 1:3358786/71‑1 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGgga < 1:556498/71‑1 (MQ=255) tCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTgg < 1:3697883/58‑1 (MQ=255) tCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTgg < 1:4256397/58‑1 (MQ=255) tCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTgg < 1:866629/58‑1 (MQ=255) tCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTgg < 1:1227673/58‑1 (MQ=255) ccTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattg > 1:226292/1‑71 (MQ=255) cTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattgg < 1:1041713/71‑1 (MQ=255) cTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattgg < 1:1604559/71‑1 (MQ=255) cTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattg > 1:1883784/1‑70 (MQ=255) cTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattg > 1:3163063/1‑70 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGagag < 1:4592/54‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGagag < 1:544862/54‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttact < 1:1808628/71‑1 (MQ=255) cTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGga < 1:1507705/49‑1 (MQ=255) cTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGga < 1:1983927/49‑1 (MQ=255) tGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttactt < 1:1666934/69‑1 (MQ=255) gATAAGCGTAGCGCATCAGGCAATTAATCCCACCCTCTCCACATGgagaa > 1:977454/1‑49 (MQ=18) gATAAGCGTAGCGCATCAGGCAATTAATCCCACCCTCTCCACATGGAGAagg > 1:4367440/1‑52 (MQ=17) gATAAGCGTAGCGCATCAGGCAATTAATCACACCCTCTCCACATGGAGAagg > 1:3493153/1‑52 (MQ=17) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTg < 1:4012893/53‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttacttac < 1:2134512/69‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttacttac < 1:2823364/69‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttact < 1:633058/66‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACg < 1:1377683/70‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACg < 1:1199479/70‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACg < 1:2495068/70‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACg < 1:3929811/70‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa < 1:1307737/71‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa < 1:626989/71‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa < 1:2284931/71‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa < 1:2847608/71‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa > 1:2738317/1‑71 (MQ=255) tAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGCGAGGGTGGGATTGGATTACTTACGAc < 1:558813/71‑1 (MQ=255) tAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGAc < 1:2293646/71‑1 (MQ=255) aGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAggg > 1:607228/1‑48 (MQ=255) aGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAggg > 1:1040382/1‑48 (MQ=255) aGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttac > 1:3594054/1‑49 (MQ=255) aGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGAc > 1:3279558/1‑56 (MQ=255) aGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTcc < 1:3560561/70‑1 (MQ=255) ggCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTcc < 1:262613/69‑1 (MQ=255) ggCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:2390378/71‑1 (MQ=255) ggCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:927702/71‑1 (MQ=255) ggCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:2212452/71‑1 (MQ=255) gCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:61595/70‑1 (MQ=255) aTTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACt > 1:2306916/1‑71 (MQ=255) aaCCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCa > 1:4344839/1‑71 (MQ=255) aaCCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCa > 1:2728458/1‑71 (MQ=255) aCCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCAt < 1:918401/71‑1 (MQ=255) cccACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTc < 1:4164105/67‑1 (MQ=255) ccACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATcc > 1:2539323/1‑70 (MQ=255) cACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCAc < 1:1849186/71‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa > 1:2198380/1‑41 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa > 1:288898/1‑41 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa > 1:1064229/1‑41 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:1770434/58‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:1198366/58‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:1547238/58‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACg < 1:2705384/71‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATT‑GATTACTTACGACCTGCcagc < 1:2191409/49‑1 (MQ=255) ccTTCTCCATGTGGAGAGGGTGGGATTGGAttacttac > 1:830441/1‑38 (MQ=255) ccTTCTCCATGTGGAGAGGGTGGGATTGGAttactta > 1:1029954/1‑37 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:3036982/1‑58 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:1621516/1‑58 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:864015/1‑58 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:637194/1‑58 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:1082365/1‑58 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACt < 1:1898190/60‑1 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCAc < 1:1863717/68‑1 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCt < 1:1195300/71‑1 (MQ=255) ttCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCt > 1:1362051/1‑41 (MQ=255) ttCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCtg > 1:4141888/1‑71 (MQ=255) tctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:2680011/1‑56 (MQ=255) tctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:3746820/1‑56 (MQ=255) tctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:3821952/1‑56 (MQ=255) tctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:4154295/1‑56 (MQ=255) ctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:3418365/1‑55 (MQ=255) ctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:4029507/1‑55 (MQ=255) cATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTggtggt < 1:3642522/71‑1 (MQ=255) cATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTggtggt < 1:3208455/71‑1 (MQ=255) | AACAAATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTGGTGGT > minE/2567191‑2567328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |