| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,726,579 | T→G | 6.5% | S168S (TCA→TCC) | yheS ← | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,726,579 | 0 | T | G | 6.5% | 211.5 / 10.0 | 77 | S168S (TCA→TCC) | yheS | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base T (32/40); new base G (3/2); total (35/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01 | |||||||||||
CCATTTTTCCAGCCAGATAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACC > minE/2726510‑2726645 | ccATTTTTCCAGCCAGATAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTg < 1:3323642/71‑1 (MQ=255) tttCCAGCCAGATAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACgg > 1:1953250/1‑71 (MQ=255) tttCCAGCCAGATAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACgg > 1:1165499/1‑71 (MQ=255) tttCCAGCCAGATAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACgg > 1:2155117/1‑71 (MQ=255) tttCCAGCCAGATAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACgg > 1:4025726/1‑71 (MQ=255) ttCCAGCCAGATAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCt > 1:1342854/1‑64 (MQ=255) ttCCAGCCAGATAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCt > 1:3216140/1‑64 (MQ=255) ccagATAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCa < 1:1410610/71‑1 (MQ=255) ccgATACCGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCa < 1:1457253/68‑1 (MQ=255) cataTAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAg > 1:1769894/4‑71 (MQ=255) cagATAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAg > 1:707835/1‑71 (MQ=255) aTAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTg < 1:1192800/55‑1 (MQ=255) aTAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTg < 1:3436228/55‑1 (MQ=255) aTAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTg < 1:3739407/55‑1 (MQ=255) tAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTg < 1:20068/54‑1 (MQ=255) aaCGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCt > 1:4290818/1‑71 (MQ=255) aCGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTg > 1:3580068/1‑71 (MQ=255) ggCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGaa > 1:21191/1‑52 (MQ=255) ggCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGaa > 1:170956/1‑52 (MQ=255) ggCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGaa > 1:4002062/1‑52 (MQ=255) ggCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGaa > 1:3169035/1‑52 (MQ=255) ggCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGaa > 1:2430409/1‑52 (MQ=255) ggCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGaa > 1:3410204/1‑52 (MQ=255) ggCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTggg > 1:1460593/1‑71 (MQ=255) gCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTggg > 1:3071159/1‑70 (MQ=255) gCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGc < 1:1431423/71‑1 (MQ=255) gCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGc < 1:3104206/71‑1 (MQ=255) gCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGc < 1:762173/71‑1 (MQ=255) tcgagatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTg < 1:908211/46‑1 (MQ=255) gagatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGaa > 1:123450/1‑46 (MQ=255) gagatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCaa < 1:577013/68‑1 (MQ=255) gagatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCaa < 1:1391996/68‑1 (MQ=255) gagatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCaa < 1:2919074/68‑1 (MQ=255) gagatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGt < 1:3586244/71‑1 (MQ=255) agatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGtt < 1:915776/71‑1 (MQ=255) agatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGt > 1:777171/1‑70 (MQ=255) agatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGt > 1:2544637/1‑70 (MQ=255) atcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACg < 1:2719427/45‑1 (MQ=255) atcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTaa < 1:592740/71‑1 (MQ=255) atcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTaa < 1:1432369/71‑1 (MQ=255) tcgagGTGGTTGGTCGGTTCGTCGAGCAACAGCAGGTCGGACGGGCACATCAGCGCCTGCGCCAGGtt > 1:891876/1‑68 (MQ=12) tcgagGTGGTTGGTCGGTTCGTCGAGCAACAGCAGGTCGGACGGGCACATCAGCGCCTGCGCCAGGtt > 1:698128/1‑68 (MQ=12) tcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAg < 1:2313079/71‑1 (MQ=255) cgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGc > 1:1042042/1‑45 (MQ=255) cgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGc > 1:3591240/1‑45 (MQ=255) cgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGc > 1:3517874/1‑45 (MQ=255) gagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGa > 1:2821488/1‑70 (MQ=255) gagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGa < 1:3818389/70‑1 (MQ=255) ggtggtTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGaa < 1:1269891/38‑1 (MQ=255) gtggtTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:2489140/71‑1 (MQ=255) gtggtTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa > 1:3172757/1‑71 (MQ=255) gtggtTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:3978063/71‑1 (MQ=255) ggtTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:1879184/1‑71 (MQ=255) gtTGGTCGGTTCGTCGAGCAGCAGCAGGTCGGAAGGGCACATCAGCGCCTGCGCCAGGTTCAGGCg < 1:3835698/66‑1 (MQ=12) gtTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATAc < 1:3482446/71‑1 (MQ=255) aGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGa > 1:658986/1‑61 (MQ=255) aGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGa > 1:2321576/1‑61 (MQ=255) aGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGc > 1:4308151/1‑70 (MQ=255) cgtcgAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTaa > 1:2524353/1‑51 (MQ=255) tcgAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCgg > 1:1448014/1‑70 (MQ=255) gAGCAGCAGCAGGTCGGAAGGGCACATCAGCGCCTGCGCCAGGTTCAGGCGCATGCGCCAGCCGCCCGag > 1:1745326/1‑69 (MQ=255) gAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:53758/53‑1 (MQ=255) agcagcagcAGGTCGGAAGGGCACATCAGCGCCTGCGCCAGGTTCAGGCGCATGCGCCAGCCGCCCGagaa < 1:2994622/71‑1 (MQ=255) cagcagcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAAt < 1:3866905/71‑1 (MQ=255) agcagcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATc < 1:457950/71‑1 (MQ=255) agcagcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATc < 1:2414877/71‑1 (MQ=255) agcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACt < 1:1470357/71‑1 (MQ=255) agcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACt < 1:3975827/71‑1 (MQ=255) agcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACt < 1:1044827/71‑1 (MQ=255) gcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACtt < 1:1021921/71‑1 (MQ=255) aGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTAcc < 1:2741484/71‑1 (MQ=255) gTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCgg < 1:3578605/56‑1 (MQ=255) tCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACg < 1:2686455/38‑1 (MQ=255) tCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACg < 1:1957139/38‑1 (MQ=255) cTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:3165588/39‑1 (MQ=255) cTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:679759/39‑1 (MQ=255) cTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:3807066/39‑1 (MQ=255) cTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:4431460/1‑41 (MQ=255) cTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:1769781/1‑41 (MQ=255) tGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa > 1:2938612/1‑38 (MQ=255) | CCATTTTTCCAGCCAGATAACGGCATCGAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACC > minE/2726510‑2726645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |